| TFome pUT6483 | |
|---|---|
| Template | BT067948.1 |
| Genbank Insert ID | KJ728207 |
| Protein Name | ZmPHD38 |
| Species | Zea mays |
| TF Family | PHD |
| Gene id | GRMZM2G473258 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.Stop+267. Additional exon not accounted for in model. Supported by template FlcDNA and RNA-seq. c.G421T p.A141S Not in functional domain. Not present in template FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT6483
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B554_F |
| Temperature (ºC) | 65.7 |
| Sequence
|
caccATGGCTGTAAAATCTAGTAGAAGTTCAG |
3' Primer
| Name | B554_R |
| Temperature (ºC) | |
| Sequence
|
caatgaatataccttatttatgtacttcag |
PCR Condition
PCR Conditions: Volumes per reaction (25l): 5 l GC
Amino Acid Sequence
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MAVKSSRSSDESYIEPLLCWKKEFRSSVIKIVDSSLNSSLMSADSICSIPASEYGFYFNKRMKMDEEYDHLLTNGNMKDSSTANFTTTGYVSSPERNMDGQSCMVSSTTEFLAGSHSNVNTGQPSSSSSAPSYISQSIKESWECSLPDTNTPKPLTELISARDLCISILKRKICSAKDSELSRTSSTIDRDDNLNNPLFECTKCGLMEDPSKMLICDCCEGAFHLLCCNPHVKKIPEEEWYCLACKRKKPKRQRGKFANPKVESSKGIQRPRRGLGPIRDMLLDSESYESGVRIGTKFQAEVPKWSGPISSREDEFAEPTELDRSKTTMLSFEDKKTSIGNWIQCREVLDTGVVCGKWRRAPLFVVQSSDWDCSCSVVWDPIHADCAVPQELETVKVHEQLKYINKVYSL
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCTGTAAAATCTAGTAGAAGTTCAGATGAAAGCTATATAGAGCCTCTCCTATGCTGGAAGAAGGAATTCAGATCATCTGTAATTAAAATTGTTGATTCGTCCTTAAACTCGAGTTTGATGTCTGCCGACAGTATTTGTTCAATTCCTGCTTCAGAATATGGTTTCTATTTTAATAAACGAATGAAGATGGATGAGGAATATGATCACCTTCTGACAAATGGAAATATGAAAGATAGTAGCACGGCAAACTTCACAACCACTGGATATGTCTCATCTCCAGAGCGCAACATGGATGGTCAATCATGTATGGTTAGTTCAACTACAGAATTTTTAGCAGGATCACATTCTAATGTTAATACTGGTCAGCCATCATCCTCATCAAGTGCACCATCTTATATCAGTCAGAGTATTAAAGAATCTTGGGAGTGTTCATTGCCTGATACAAATACTCCAAAACCATTAACAGAGTTAATATCAGCGAGGGACCTTTGCATCTCCATCCTCAAAAGGAAAATATGTTCCGCCAAAGACTCAGAACTAAGCAGAACATCAAGTACTATAGACCGTGATGATAATCTAAACAATCCTTTATTTGAATGCACAAAATGCGGTCTGATGGAAGATCCATCCAAAATGTTAATTTGTGATTGTTGTGAAGGAGCATTTCATTTATTGTGTTGCAATCCACATGTCAAGAAAATACCAGAGGAAGAGTGGTATTGTTTGGCATGTAAAAGAAAGAAACCTAAGAGACAACGTGGAAAGTTTGCAAATCCCAAAGTTGAATCATCCAAGGGCATTCAAAGACCCCGCCGAGGTCTTGGTCCTATTCGAGATATGTTACTGGATTCTGAATCATATGAAAGTGGGGTTCGGATTGGTACTAAGTTTCAAGCAGAAGTTCCAAAATGGTCTGGTCCTATTTCTAGTAGAGAAGATGAGTTTGCTGAGCCCACTGAACTTGATCGTAGTAAAACGACAATGCTGAGTTTCGAGGATAAGAAAACCAGTATCGGTAACTGGATCCAGTGCCGGGAAGTTCTAGACACAGGAGTTGTTTGTGGCAAATGGCGGAGGGCACCATTGTTTGTTGTTCAATCAAGTGACTGGGACTGTTCGTGCTCAGTTGTTTGGGATCCTATCCATGCTGATTGTGCTGTTCCACAGGAATTGGAGACCGTTAAAGTTCACGAGCAACTGAAGTACATAAATAAGGTATATTCATTG
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