TFome clone pUT6501

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C516_F
Temperature (ºC)	70.9
Sequence Copy to clipboard	caccATGGACCCGGACTTCTCGCC

3' Primer

Name	C516_R
Temperature (ºC)
Sequence Copy to clipboard	CGTAGGTTGCTTTATCGTAAGCT

PCR Condition

5 l GC buffer (Thermo Fisher) 1 l primer forward 2

Amino Acid Sequence

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MDPDFSPGGGGPSFEFAFNEVNFSDRELRIEVVAGDDYAPGSSGAGAGGGGLADWARHRKRRREELLKEKESTTHMSDQTNCNEVEAEECDAYEENQEEPVAMVEESPPDVGQDGDDGQGIDSSWTVVGTPVLRVKTIYISSAILAAKSPFFFKLFSNGMKESDQRHATLRITDSEETALMELLSFMYSGKLATTEPTLLLDILMAADKFEVVSCMRYCSQLLTSLPMTTESALLYLDLPCSISMAAAVQPLTDAAKDFLAVKYKDLTKFQDEVMNIPLAGIEAILSSNDLQVASEDTIYDFLLRWARAQYPKSEERREILSSRLLPLVRFSHMTCRKLRKVLTCTDIDHEQATKCVTEALLYKADAPHRQRALAADTTTCRKFAERAYKYRPLKVVEFDRPYPQCIAYLDLKREECSRLFPAGRIYSQAFHLAGQGFFLSAHCNMEQQSTFYCFGLFLGMQEKGSTSVTVDYEFAARTRPSGEFVSKYKGNYTFTGGKAVGYRNLFGIPWQAFMADDSLFFIDGMLHLRAELTIKQPT

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACCCGGACTTCTCGCCCGGGGGCGGGGGGCCCAGCTTTGAATTCGCCTTCAACGAGGTCAACTTCTCCGACCGGGAATTGCGTATCGAGGTCGTCGCCGGGGATGACTACGCTCCGGGGTCCAGCGGCGCCGGTGCCGGAGGAGGTGGCCTCGCCGACTGGGCGCGCCACCGCAAGCGCCGCCGTGAGGAGCTCCTCAAGGAGAAAGAATCTACAACTCACATGTCAGACCAAACAAATTGCAATGAAGTTGAAGCAGAAGAGTGTGATGCGTATGAAGAAAATCAAGAGGAACCTGTAGCAATGGTAGAAGAATCTCCACCCGATGTTGGCCAAGATGGTGATGATGGGCAAGGTATTGATTCGTCTTGGACTGTGGTTGGTACACCAGTTTTACGAGTCAAGACAATTTATATCAGTTCAGCGATTCTTGCTGCGAAGAGTCCTTTCTTTTTCAAGCTTTTCTCTAACGGCATGAAAGAATCGGATCAGAGACATGCAACCCTTAGAATTACTGACTCAGAGGAAACTGCCCTCATGGAGCTTTTAAGCTTTATGTATAGTGGAAAGTTGGCAACTACTGAGCCCACTCTTCTGCTGGATATCTTGATGGCTGCCGACAAATTTGAGGTTGTTTCATGCATGAGGTACTGCAGCCAGTTGCTCACAAGCTTGCCGATGACCACAGAATCTGCACTTCTCTACCTAGATCTCCCTTGCTCAATTTCAATGGCAGCAGCAGTTCAACCTCTGACAGATGCAGCTAAGGATTTCCTTGCTGTAAAATACAAGGATTTGACTAAGTTCCAAGATGAAGTGATGAACATTCCTCTTGCTGGAATTGAAGCCATCTTGTCAAGTAATGACCTCCAGGTGGCATCTGAAGATACCATCTATGACTTCTTGCTCAGATGGGCCCGTGCACAATACCCGAAATCAGAAGAGAGACGTGAGATCCTTAGTTCTCGGCTGCTCCCTCTTGTGCGATTCAGTCACATGACATGTAGGAAGCTGCGGAAGGTCCTAACATGCACAGATATAGACCACGAGCAGGCAACCAAGTGTGTCACCGAGGCTCTTCTGTACAAAGCTGATGCACCACACCGGCAACGAGCTCTTGCAGCGGACACAACAACCTGTCGGAAATTCGCTGAGCGGGCTTACAAGTACAGACCACTCAAGGTCGTTGAGTTTGATCGGCCCTATCCACAGTGCATAGCATACTTGGATCTCAAGCGCGAGGAGTGCTCTCGACTCTTTCCTGCAGGCCGGATATACTCGCAAGCGTTCCACCTCGCAGGGCAGGGCTTCTTCCTCTCAGCTCACTGTAACATGGAGCAGCAAAGCACCTTCTACTGCTTTGGCCTGTTCCTGGGGATGCAAGAGAAGGGCTCAACGAGCGTGACCGTGGACTATGAGTTTGCTGCAAGGACGAGACCATCCGGCGAGTTTGTGAGCAAGTATAAGGGTAACTACACATTTACCGGTGGGAAGGCAGTTGGGTACAGGAATCTCTTTGGAATCCCGTGGCAGGCGTTCATGGCCGATGACAGCCTCTTCTTCATCGACGGGATGCTGCATCTGAGAGCTGAGCTTACGATAAAGCAACCTACG