| TFome pUT6527 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728250 |
| Protein Name | ZmHB66 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G351330 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6527
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0087_F |
| Temperature (ºC) | 69.2 |
| Sequence
|
caccATGGACAGGCCAGATCACCAG |
3' Primer
| Name | CD0087_R |
| Temperature (ºC) | |
| Sequence
|
GGCCACCGCATTCCACTCAAC |
PCR Condition
PCR Reaction Mix:5 l GC buffer (Thermo Fisher) 1 l
Amino Acid Sequence
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MDRPDHQQQQFFMPTTVQVPQPQQQQQQLCVPMLDEPPSSFLAGRGGGGASGRGERKRRFTEEQIRSLESMFHAHHAKLEPREKAELARELGLQPRQVAIWFQNKRARWRSKQLEHDYALLRAKFDDLHAHVESLKQDKLALTTQLSELSERLRERDDRAAAAGGGGRETMASSSSCIGGGGEEEAEDDKRNVLLFGCVDMEPPAESCVLVGSTCAALADVSVESECDDQHLHYDDEFPESYCAMPELWEPWPLVEWNAVA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACAGGCCAGATCACCAGCAGCAGCAGTTCTTCATGCCGACGACGGTGCAGGTGCCACAGCCACAGCAGCAGCAGCAGCAGCTCTGCGTCCCGATGCTCGACGAGCCGCCGTCCTCGTTTTTGGCGGGGAGAGGCGGAGGCGGGGCGTCGGGGAGGGGGGAGAGGAAGCGGCGGTTCACGGAGGAGCAGATACGGTCGCTGGAGTCCATGTTCCACGCGCACCACGCCAAGCTGGAGCCCCGGGAGAAGGCGGAGCTGGCGCGGGAGCTGGGCCTGCAGCCGCGGCAGGTGGCCATCTGGTTCCAGAACAAGCGCGCCCGGTGGCGCTCCAAGCAGCTCGAGCACGACTACGCCCTGCTCCGCGCCAAGTTCGACGATCTCCACGCCCACGTCGAGTCCCTCAAGCAGGACAAGCTCGCACTCACCACTCAGTTGAGCGAGCTAAGCGAGAGGCTGAGGGAGCGGGACGACCGGGCCGCCGCCGCCGGCGGCGGCGGCCGCGAGACGATGGCTAGCAGCAGCAGCTGCATCGGCGGTGGTGGTGAGGAGGAGGCGGAGGACGACAAGAGGAACGTCCTGCTGTTCGGATGCGTCGACATGGAGCCGCCCGCCGAGAGCTGTGTGCTCGTCGGGTCGACGTGCGCGGCGCTGGCCGACGTCTCGGTGGAGTCCGAGTGCGACGACCAGCACCTTCACTACGACGACGAGTTCCCGGAGTCCTACTGCGCCATGCCGGAGCTGTGGGAGCCCTGGCCGCTTGTTGAGTGGAATGCGGTGGCC
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