TFome clone pUT6528

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	CD0354_F
Temperature (ºC)	67.4
Sequence Copy to clipboard	caccATGGTATGCATCGGTAGCCTC

3' Primer

Name	CD0354_R
Temperature (ºC)
Sequence Copy to clipboard	TCCGGTTTTGTCATGGTGAAATTG

PCR Condition

Volumes per reaction (25l): 6 l GC buffer (Thermo

Amino Acid Sequence

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MVCIGSLLRALVLSAAASAGRRRGPRILCGHEADVRVTPRHGHGWRGFRAVTGRMMLDSSDCASAAAAAETGQVQPQRRAVGAVPLAHDGGYASGGWEREDGKLSCGYSSFRGKRATMEDFYDVKLTEIDGQAVSLFGVFDGHGGSRAAEYLREHLFDNLLKHPDFLTDTKLAISETYQKTDTDFLESEASAFRDDGSTASTALLVGDHLYVANVGDSRAVISKAGKAMALSEDHKPNRIDERKRIENAGGIVIWAGTWRVGGVLAMSRAFGNRLLKPYVVAEPEIQEEQVSGGLECLVLASDGLWDVVENEEAVFLGRSEDTPESAARKLTEIAYSRGSADNITCIVVQFHHDKTG

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGTATGCATCGGTAGCCTCCTGAGGGCGCTGGTCCTGAGTGCGGCGGCGTCTGCGGGCAGGCGCCGGGGGCCCAGGATCCTGTGTGGCCACGAGGCGGATGTACGCGTGACTCCTCGGCACGGACACGGCTGGCGCGGATTCCGGGCAGTCACGGGAAGGATGATGCTGGACTCCTCTGACTGTGCCTCTGCTGCCGCCGCCGCGGAGACGGGGCAGGTGCAGCCGCAGCGCCGCGCGGTGGGGGCCGTGCCCCTGGCGCACGACGGCGGCTACGCCAGCGGCGGATGGGAGAGAGAAGATGGCAAACTGAGTTGTGGATACTCAAGTTTTAGAGGCAAGAGAGCTACGATGGAAGATTTCTATGATGTAAAACTAACTGAAATTGATGGACAAGCTGTTAGCCTGTTTGGTGTTTTCGACGGTCATGGAGGATCACGTGCTGCTGAGTATTTGAGGGAGCACTTGTTTGATAACCTTCTCAAGCACCCTGATTTCTTGACAGATACAAAGCTTGCGATAAGTGAAACATATCAGAAAACAGATACAGATTTCTTGGAATCAGAAGCAAGCGCTTTCAGGGATGATGGTTCAACAGCTTCAACTGCACTTTTGGTGGGTGACCATTTATATGTTGCAAATGTTGGTGATTCTCGTGCCGTTATTTCAAAAGCTGGCAAAGCTATGGCGCTTTCAGAAGATCACAAACCTAACAGAATTGATGAGCGAAAGAGAATTGAAAATGCTGGTGGTATTGTCATTTGGGCTGGCACTTGGAGGGTGGGTGGTGTATTGGCAATGTCCCGTGCATTTGGCAATCGTTTACTGAAGCCATATGTGGTGGCAGAACCTGAAATTCAGGAAGAACAGGTCAGTGGTGGGCTGGAATGCCTGGTTCTTGCTAGTGATGGGCTTTGGGATGTTGTGGAAAATGAGGAAGCTGTTTTCCTTGGGAGATCGGAGGACACGCCAGAGTCTGCAGCTAGGAAATTGACAGAGATTGCTTACTCTCGCGGCAGCGCTGACAATATCACATGCATCGTGGTCCAATTTCACCATGACAAAACCGGA