| TFome pUT6529 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728252 |
| Protein Name | ZmALF5 |
| Species | Zea mays |
| TF Family | Alfin-like |
| Gene id | GRMZM2G016817 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6529
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0331_F |
| Temperature (ºC) | 71.2 |
| Sequence
|
caccATGGACCCCGGCGCC |
3' Primer
| Name | CD0331_R |
| Temperature (ºC) | |
| Sequence
|
GCAAAGTGACTGTCGGCG |
PCR Condition
PCR Reaction Mix:5 l GC buffer (Thermo Fisher) 1 l
Amino Acid Sequence
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MDPGAGAHYSARTAEEVFRDFRGRRAGMIKALTNDVEKFYQLCDPGESARPPRGRRQSLC
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGACCCCGGCGCCGGTGCACACTACTCCGCCCGTACCGCGGAGGAAGTCTTCCGCGATTTCCGCGGCCGCCGCGCGGGCATGATCAAGGCCCTCACCAACGATGTGGAGAAGTTCTACCAGCTGTGCGACCCCGGTGAGTCTGCTCGACCTCCTCGGGGCCGCCGACAGTCACTTTGC
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