| TFome pUT6552 | |
|---|---|
| Template | BT054562 |
| Genbank Insert ID | KJ728274 |
| Protein Name | ZmMTERF21 |
| Species | Zea mays |
| TF Family | mTERF |
| Gene id | GRMZM2G161146 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.A360G p.Gln120(=), c.A758G p.E253G Present in functional domain; Present in template FlcDNA BT054562 |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6552
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C482F |
| Temperature (ºC) | 67.4 |
| Sequence
|
caccATGCTGCGCCTTCAACATC |
3' Primer
| Name | C482R |
| Temperature (ºC) | |
| Sequence
|
ATCTTCATGAGGCATTTTCCCAGC |
PCR Condition
Step 1, 98°C 40 sec melt, 1 cycle; Step 2, 98&
Amino Acid Sequence
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MLRLQHHFLSSIGASSHRHCSLLSIATTAITPDRFVAEEYLVTTCGLTPAQAGRASKGLTHLKSPVKPDAVVAFLAGVGLAKDDIIAGIARYPRLLCSKVDKTLTPRFAQLMSLGLSPSQISRLITIVPNIFVAPKKISHLQFYLSFMGCFDRVHSAIRINPMLLSRNLEDVVKPNIAFLLQCGLTVSNVLEFPLLIGMRPESVRERVACAEKLGVPRNTGMFKSALWAVYCVGPNSIGAKLDVIKATLGCSGAELTLVVRKSPPILRMSEGKLSRALKFLKVDVGLKLQYILLRPAILAFSMQRRLMPRHYFIKILKAKGLVKENVDFYNALCLTEKRFAQKFIDPYNQSIAGLADAYAAACAGKMPHED
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCTGCGCCTTCAACATCATTTCTTGTCCTCCATCGGCGCGTCCTCCCATCGCCACTGCAGCCTCCTCTCTATTGCTACCACTGCCATTACTCCCGATCGATTCGTCGCTGAGGAATACCTGGTCACCACCTGCGGGCTCACCCCAGCGCAAGCGGGCAGGGCGTCCAAGGGGCTCACCCACCTCAAGTCTCCGGTTAAGCCCGACGCCGTCGTCGCCTTTCTCGCGGGCGTCGGTCTCGCCAAAGACGACATCATCGCCGGTATCGCCAGGTACCCGCGGCTCCTCTGCTCCAAGGTGGACAAAACCCTAACCCCTCGCTTCGCTCAGCTCATGAGCCTTGGGCTATCCCCTTCCCAGATCTCCCGCCTCATTACCATCGTCCCCAACATCTTCGTCGCTCCCAAAAAGATCTCCCACCTCCAGTTCTATCTCTCCTTCATGGGTTGCTTCGATCGGGTTCACTCCGCCATCAGGATCAATCCAATGCTCCTCAGTCGGAACCTTGAGGATGTGGTCAAGCCTAACATAGCATTCCTACTGCAGTGCGGTCTAACTGTTTCTAATGTTCTAGAGTTCCCATTGCTGATAGGCATGAGGCCCGAGAGTGTCAGGGAAAGGGTGGCATGCGCTGAGAAGCTTGGTGTGCCGCGCAATACAGGGATGTTCAAGAGTGCCCTGTGGGCCGTCTATTGCGTTGGTCCCAACTCCATTGGTGCAAAATTGGATGTCATCAAGGCGACTCTTGGATGCTCTGGGGCGGAGCTGACCCTTGTGGTGCGCAAGTCACCTCCAATTCTGAGGATGTCAGAAGGCAAGCTGAGTCGCGCATTGAAGTTCCTGAAGGTGGATGTTGGGTTGAAGCTTCAGTACATCTTGCTTAGGCCAGCAATACTTGCCTTTAGCATGCAGAGGCGGCTGATGCCCAGACATTATTTCATCAAGATTCTGAAGGCGAAGGGGCTTGTGAAGGAAAATGTTGACTTTTATAATGCTCTTTGTTTAACTGAAAAGAGATTTGCCCAGAAGTTTATTGATCCTTACAACCAGAGTATTGCAGGGCTTGCAGATGCTTATGCTGCTGCTTGTGCTGGGAAAATGCCTCATGAAGAT
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