TFome clone pUT6562

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	P31up
Temperature (ºC)	65.3
Sequence Copy to clipboard	caccATGGGAAGAAGCGAAGGCAGCAC

3' Primer

Name	P31dn
Temperature (ºC)
Sequence Copy to clipboard	GAAGCTTGGCACGACCCCGTAG

PCR Condition

Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98&d

Amino Acid Sequence

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MGRSEGSTSPAAGGAACACAVCGGAAVVYCAADAAALCSPCDTAVHAANLLASRHERVPLSMVTAASGVYDDDLFAPDDIDAASSWASAPAQGQGSPQNGSSSASFTTGDSGAEGRGLFDLLSDVDFAACVTGGGGYLPDGVAPVVHHGAAPLWAQPGLAAAAWTATWSPAVVVPGAAVVAAAAAEREARVQRYREKRERRKFQKTIRYASRKAYAEARPRIKGRFVKRAAGTSSSSSSGAGTSDSIDAAAKFWLSFSDDARDGFFVDAGAYGVVPSF

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGGAAGAAGCGAAGGCAGCACAAGCCCAGCGGCTGGGGGAGCCGCCTGCGCGTGCGCGGTCTGCGGCGGCGCCGCGGTGGTGTACTGCGCGGCGGACGCTGCGGCGCTCTGCAGCCCCTGCGACACCGCTGTGCACGCTGCGAACCTGCTGGCGTCGCGCCACGAGCGCGTGCCCCTTTCCATGGTGACTGCTGCATCCGGCGTGTACGACGACGACCTCTTCGCGCCCGACGACATCGACGCGGCGTCGTCGTGGGCGTCGGCGCCTGCGCAGGGGCAGGGAAGCCCCCAGAACGGGAGCTCGTCGGCGAGCTTCACCACCGGCGACAGCGGCGCGGAGGGCAGGGGCCTGTTCGACCTGCTGTCCGACGTCGACTTCGCTGCCTGTGTCACGGGCGGCGGCGGCTACCTGCCGGACGGCGTGGCGCCCGTCGTCCATCACGGCGCTGCACCTCTGTGGGCGCAGCCCGGTCTGGCCGCCGCCGCATGGACGGCCACGTGGTCGCCCGCTGTCGTCGTCCCGGGCGCCGCCGTGGTCGCCGCGGCGGCGGCGGAGCGGGAGGCGAGGGTGCAGAGGTACCGCGAGAAGCGCGAGAGGCGCAAGTTCCAGAAGACCATCCGCTACGCGTCCCGCAAGGCCTATGCCGAGGCGCGGCCCAGGATCAAGGGCCGCTTTGTCAAGCGCGCCGCCGGCACCTCCTCCTCCTCCTCCTCGGGTGCCGGCACATCGGACAGCATCGACGCTGCTGCCAAGTTCTGGCTCTCCTTCTCCGACGACGCCAGGGACGGCTTCTTCGTGGACGCCGGGGCCTACGGGGTCGTGCCAAGCTTC