| TFome pUT6570 | |
|---|---|
| Template | BT038417 |
| Genbank Insert ID | KJ728291 |
| Protein Name | ZmOrphan309 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G398615 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT6570
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B965_F |
| Temperature (ºC) | 68.0 |
| Sequence
|
caccATGGGGCGTGTAAATGGAAGTTTA |
3' Primer
| Name | B965_R |
| Temperature (ºC) | |
| Sequence
|
CTGGGTCTCAGAGCATCCC |
PCR Condition
5 l GC buffer (Thermo Fisher) 1 l primer forward 2
Amino Acid Sequence
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MGRVNGSLNLSRAIGDMELKQNKFLSPDKQILTANPDINIVELCDDDEFIVLACDGIWDCMSSQQLVDFIREHINTEESLSAVCERVLDRCLAPSTAGEGCDNMTMILVQFKKPFAQVKDASGAEQLTGDAGCSETQ
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGCGTGTAAATGGAAGTTTAAATTTGTCAAGAGCTATTGGAGACATGGAGCTTAAACAAAACAAGTTCTTGTCCCCTGATAAGCAAATTTTGACCGCAAACCCTGACATAAACATTGTCGAGCTATGTGACGACGACGAATTCATTGTTTTGGCATGTGATGGCATTTGGGACTGCATGTCAAGCCAGCAGTTGGTTGATTTCATCCGTGAACATATAAACACAGAGGAAAGCCTTTCTGCAGTATGTGAAAGAGTGCTTGATAGATGCCTGGCTCCATCGACGGCTGGCGAGGGATGCGATAACATGACGATGATCCTGGTGCAGTTCAAGAAGCCATTTGCTCAGGTAAAGGATGCCAGTGGTGCGGAACAACTAACTGGAGATGCGGGATGCTCTGAGACCCAG
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