| TFome pUT6571 | |
|---|---|
| Template | BT069933 |
| Genbank Insert ID | KJ728292 |
| Protein Name | ZmCA2P10 |
| Species | Zea mays |
| TF Family | CCAAT-HAP2 |
| Gene id | GRMZM2G165488 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C399T p.(=). In template FlcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6571
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B967_F |
| Temperature (ºC) | 67.9 |
| Sequence
|
caccATGATGAGCTTCAAGGGACACG |
3' Primer
| Name | B967_R |
| Temperature (ºC) | |
| Sequence
|
CGCCCTGAGGAGCTCG |
PCR Condition
5 l GC buffer (Thermo Fisher) 1 l primer forward 2
Amino Acid Sequence
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MMSFKGHEGFGQVSGAGMSQASHGAAPAGAPLPWWAGAQLLSGEPAPLSPEEAPRDTQFQVVPGASQGTPDPAPPKGGTPKVLKFSVFQGNLESGGKGEKTPKNSTAVVLQSPFAEYNGRFEIGLGQSMLVPSSYSCADQCYGMLTTYGMRSMSGGRMLLPLIAPADAPVYVNPKQYEGILRRRRARAKAESENRLTKGRKPYLHESRHLHAMRRVRGSGGRFLNTNKGGHGTDVAANGGSKMAAAAAPSRLAMPPSAEPPWLSGLSDGSNPCCHSRSSVSSLSGSYVASIYGGLEQHLRAPPFFTPLPPVMDGDHGGPTAATISSFKWAASDGCCELLRA
TFome clones do not contain stop codon at 3'end.
CDS
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ATGATGAGCTTCAAGGGACACGAGGGGTTCGGTCAGGTGTCCGGAGCCGGGATGAGCCAGGCCTCCCATGGCGCCGCGCCTGCCGGAGCCCCGCTGCCGTGGTGGGCTGGGGCCCAGCTGCTGTCCGGCGAGCCGGCGCCCCTGTCCCCGGAGGAGGCGCCCCGGGACACCCAGTTCCAGGTCGTGCCGGGGGCCTCTCAGGGCACGCCGGATCCAGCGCCGCCCAAGGGAGGGACACCTAAGGTCCTCAAGTTCTCTGTGTTCCAAGGGAATTTGGAGTCGGGTGGTAAAGGAGAGAAAACCCCAAAGAACTCTACCGCTGTCGTTCTGCAGTCGCCATTCGCGGAATACAATGGTCGTTTCGAGATCGGTCTCGGTCAATCTATGCTGGTCCCTTCTAGTTATTCTTGTGCTGACCAGTGCTATGGCATGCTTACGACTTATGGAATGAGATCCATGTCTGGTGGGAGAATGCTGTTGCCACTAATTGCGCCAGCCGATGCACCCGTTTATGTGAACCCGAAACAGTACGAAGGCATCCTCCGTCGTCGCCGTGCTCGCGCTAAGGCGGAGAGCGAGAACAGGCTCACCAAAGGCAGAAAGCCTTATCTCCATGAGTCGCGCCACCTCCACGCGATGCGCCGGGTGAGAGGCTCCGGCGGGCGCTTCCTCAACACGAATAAAGGAGGGCACGGCACGGACGTTGCTGCAAACGGGGGCAGCAAGATGGCGGCGGCGGCGGCACCATCCCGTCTCGCCATGCCCCCTAGCGCTGAGCCTCCATGGCTGTCAGGGCTCAGCGACGGCAGCAACCCGTGCTGCCACTCCCGGAGTAGTGTCTCCAGCTTGTCCGGGTCCTACGTGGCGAGCATCTACGGTGGCTTGGAGCAGCACCTCCGGGCGCCGCCCTTCTTCACCCCGCTGCCGCCCGTCATGGACGGCGACCACGGCGGCCCCACGGCCGCCACCATCTCCTCCTTCAAGTGGGCGGCCAGCGACGGCTGCTGCGAGCTCCTCAGGGCG
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