| TFome pUT6574 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728294 |
| Protein Name | ZmOrphan311 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G170201 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6574
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0387_F |
| Temperature (ºC) | 67.3 |
| Sequence
|
caccATGGGTTTCACAAGCAAGCTAAAG |
3' Primer
| Name | CD0387_R |
| Temperature (ºC) | |
| Sequence
|
GACGCTGATCCTCCCGACT |
PCR Condition
PCR Reaction Mix:5 l GC buffer (Thermo Fisher) 0.6
Amino Acid Sequence
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MGFTSKLKRSRHGVSMKLRVRDPRPTQTVQDGLSFVASLKRVFACEPDKYDEIIATMRQFKSGSIRSAVVVERVKVLLEGHPGLLRHFNQFLPWGYIRAHGPVGRISV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGTTTCACAAGCAAGCTAAAGCGCTCGAGGCATGGTGTGTCCATGAAGCTCCGAGTCCGTGATCCGAGGCCAACGCAGACAGTCCAAGATGGACTCAGTTTCGTGGCGAGCTTGAAGCGCGTGTTCGCCTGCGAGCCTGACAAGTACGACGAGATCATCGCCACCATGCGCCAGTTCAAGTCCGGGAGCATTCGTAGTGCGGTTGTGGTTGAGCGTGTGAAGGTGCTTCTGGAAGGTCACCCTGGTCTCCTCCGCCACTTCAACCAGTTCCTGCCATGGGGTTACATAAGGGCTCATGGACCAGTCGGGAGGATCAGCGTC
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