| TFome pUT6579 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728299 |
| Protein Name | ZmOrphan327 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G004483 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C171A p.(=) |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6579
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0326_F |
| Temperature (ºC) | 68.5 |
| Sequence
|
caccATGTCGGCGTCGAGCG |
3' Primer
| Name | CD0326_R |
| Temperature (ºC) | |
| Sequence
|
TGAACGGAACCATCCGAGGTC |
PCR Condition
PCR Reaction Mix:5 l GC buffer (Thermo Fisher) 1 l
Amino Acid Sequence
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MSASSAACRVCGGVAECACHGHGAGARCGAAVADLNRGFPGMWHQHHQQPEEEEPSVAGGGAAAGLQEFQFFGHDDDHDSVAWLFNDPAPHLHRGPAPAAGAAGNGVVAEAAQRRAPLFDGYAHAQYGQLPGHGLMFDVPLGRGGDDDAAVLEAGLGLGGAGAGAGDSNPATTSSATIMSFCGSTFTDAAANGSASGADPAVDREAKVMRYKEKRKRRRYEKQIRYASRKAYAEMRPRVKGRFAKVPDGDAPAPPAPAAAGGYEPGRLDLGWFRS
TFome clones do not contain stop codon at 3'end.
CDS
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ATGTCGGCGTCGAGCGCCGCGTGCCGCGTATGTGGCGGCGTAGCGGAGTGCGCGTGCCACGGCCACGGCGCCGGCGCGCGGTGCGGGGCCGCCGTCGCGGACCTCAACCGCGGGTTTCCCGGGATGTGGCACCAGCACCACCAGCAGCCGGAGGAGGAGGAGCCCAGCGTAGCCGGCGGTGGCGCGGCGGCGGGGCTGCAGGAGTTCCAGTTCTTCGGCCACGACGACGACCACGACAGCGTGGCGTGGCTGTTCAACGACCCGGCGCCCCACCTGCACCGCGGCCCGGCGCCGGCCGCGGGCGCGGCCGGGAACGGGGTTGTGGCCGAGGCCGCGCAGCGGAGAGCGCCGCTGTTCGACGGGTACGCGCACGCTCAGTACGGCCAGCTGCCGGGCCACGGGCTCATGTTCGACGTGCCGCTGGGCCGGGGCGGCGACGACGACGCGGCGGTGCTGGAGGCAGGGCTGGGGCTAGGCGGCGCCGGCGCCGGCGCCGGCGACAGCAATCCAGCGACGACGTCCAGCGCCACAATCATGTCCTTCTGCGGGAGCACGTTCACCGACGCGGCGGCCAACGGCAGCGCAAGCGGCGCCGACCCGGCAGTGGACCGGGAGGCGAAGGTGATGCGGTACAAGGAGAAGCGGAAGAGGAGGCGCTACGAGAAGCAGATTCGCTACGCTTCCCGTAAGGCGTACGCGGAGATGCGGCCGCGCGTCAAGGGCCGCTTCGCCAAGGTGCCCGACGGTGATGCTCCCGCGCCACCAGCGCCGGCCGCTGCGGGGGGCTACGAACCAGGCCGGCTCGACCTCGGATGGTTCCGTTCA
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