| TFome pUT6580 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728300 |
| Protein Name | ZmOrphan198 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G018876 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT6580
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0333_F |
| Temperature (ºC) | 67.7 |
| Sequence
|
caccATGAGGATCCAGTGCGACG |
3' Primer
| Name | CD0333_R |
| Temperature (ºC) | |
| Sequence
|
GCCAAGATCAGGAACGATGAAGTA |
PCR Condition
PCR Reaction Mix:5 l GC buffer (Thermo Fisher) 1 l
Amino Acid Sequence
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MRIQCDACEAAAAAVVCCADEAALCARCDVEIHAANKLASKHQRLPLGDAAALPASLPRCDVCQERPAFIFCVEDRALLCRDCDEPIHVPGTLSGNHQRYLATGIRVGFSSVCGASAEGLPPAPPKGSSKPTAVVSAPAAGTTTKTRTVKDALPQEVPSSPFLPPSGWAVEDLLELSDYESSDKKDSSLGLKELEWFEDIDLFHAHSPAKTAMAEFFASPQPVSNAGFYKANGARLFKKSRMEVPDDDDEDYFIVPDLG
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAGGATCCAGTGCGACGCGTGCGAGGCCGCGGCGGCCGCGGTGGTGTGCTGCGCCGACGAGGCGGCGCTGTGCGCGCGCTGCGACGTGGAGATCCACGCCGCCAACAAGCTCGCCAGCAAGCACCAGCGGCTCCCGCTCGGCGACGCCGCGGCGCTCCCCGCCAGCCTCCCGCGCTGCGACGTGTGCCAGGAGAGGCCGGCCTTCATCTTCTGCGTCGAGGACAGGGCGCTCCTCTGCCGCGACTGCGACGAGCCCATCCACGTCCCCGGCACGCTCTCCGGCAACCACCAGCGCTACCTCGCCACCGGCATCCGCGTCGGCTTCAGCTCCGTCTGCGGCGCCAGCGCCGAGGGCCTCCCGCCGGCGCCGCCCAAGGGAAGCTCCAAGCCCACGGCCGTCGTCAGCGCGCCAGCTGCCGGGACGACCACCAAAACAAGGACGGTCAAGGACGCGCTGCCGCAGGAGGTGCCGTCCTCGCCGTTCCTGCCACCGTCCGGTTGGGCCGTCGAGGACCTCCTCGAGCTGTCGGACTACGAATCCAGCGACAAGAAAGACTCGTCTCTTGGGCTCAAGGAGCTGGAGTGGTTCGAGGACATCGACCTGTTCCACGCCCATTCGCCAGCTAAAACTGCCATGGCGGAGTTCTTCGCCTCGCCGCAGCCAGTGAGTAACGCCGGGTTCTACAAGGCGAACGGGGCGCGCCTGTTCAAGAAGTCACGGATGGAGGTGCCTGACGACGACGACGAGGACTACTTCATCGTTCCTGATCTTGGC
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