| TFome pUT6583 | |
|---|---|
| Template | BT035697 |
| Genbank Insert ID | KJ728303 |
| Protein Name | ZmWRKY121 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G061408 |
| transcript | T02 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T147C p.(=). In template FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT6583
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B949_F |
| Temperature (ºC) | 65.1 |
| Sequence
|
caccATGGCGACGACGGAGACGATG |
3' Primer
| Name | B949_R |
| Temperature (ºC) | |
| Sequence
|
CCTCTTCTTGGTGTTCTTGAACG |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MATTETMEMGMGVVVDGVSSNGSGGCGGLVVTELSHIKELVRQLEVHLGGSPDLCKHLASQIFSLTERSIGLITSSSSSSGLDGARRKRSAGDAGLASPLSATPTSGVTDGPFKNTKKR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGACGACGGAGACGATGGAGATGGGCATGGGCGTCGTCGTCGACGGCGTCAGTAGCAACGGATCAGGCGGCTGCGGCGGGCTGGTGGTGACGGAGCTGAGCCACATCAAGGAGCTGGTGAGGCAGCTGGAGGTGCACCTCGGCGGCTCCCCGGACCTGTGCAAGCACCTGGCCTCGCAGATCTTCTCCCTCACCGAGCGCTCCATCGGCCTCATCACCTCCTCCTCCAGCTCCAGCGGCCTCGACGGCGCCCGCCGGAAGCGCTCCGCGGGCGACGCCGGCCTCGCCTCGCCGCTCTCGGCCACGCCCACCAGCGGCGTCACCGACGGGCCGTTCAAGAACACCAAGAAGAGG
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