| TFome pUT6587 | |
|---|---|
| Template | BT067615 |
| Genbank Insert ID | KJ728307 |
| Protein Name | ZmOrphan313 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | AC207628.4_FG011 |
| transcript | T011 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.610del45 Exon 5 skipped, which had no support in RNA-seq. Present in FlcDNA. Not in functional domain. |
| Request Information | Deposited to ABRC |
TFome clone pUT6587
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C543_F |
| Temperature (ºC) | 69.1 |
| Sequence
|
caccATGGGCAAGTCGCCGG |
3' Primer
| Name | C543_R |
| Temperature (ºC) | |
| Sequence
|
GCGCTTCCATTCAGCTTTAAGTG |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MGKSPGKWIKSVLLGKKSTKSGPTKSNESKADNNRYSTGEDRTLSESSPVISEPVLVNIHKNVAINGKAADASDRARQQDPQSQSVVESRSSAPAAQLGEDQAAAKAQAAFRGYLARRSFRALKGIVRLQALIRGYLVRRQAVSTLRATWLIVKFQALVRGRNVRLSGSRMQLNVKFGQSNFGGVRSSDAWKEKLSSNAYVRKLLSSPIVLEPLHFQYDKRDPNSTYNWFERWTIGCIWKPAFQPKRVADGKPLVKKASYAMETQSAKLKRNIRKGSAAIAGSFHTSGESDKVKRNPKNFSSFPADSVPDSQLSELEKVKRNLRKVTDSMAEASKISSSRVDSSKVCNSTAEVPKESNPVAEISKIRSLLNGISDHQDIQCENTRESSFPLGTQEDSDNDHLLRYSNMDSLDLVPGLKSDQEIQLDSVSIGENVDDPTVVAPAVEEMSPQNIDTEDNVLCKKEEARSKEEHLSNGSLRTSKRKSSFPNKSEYVENGTHATPVQPRQPSYMAATESAKAKLRAQNSPSLDSDSAAEKNGFTRRHSLPSSTKSRALKAEWKR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGCAAGTCGCCGGGGAAGTGGATCAAATCGGTGCTTTTGGGGAAGAAATCTACCAAGTCAGGTCCTACCAAGTCGAATGAATCTAAGGCTGACAACAACAGATACTCGACCGGGGAGGACCGCACATTGTCTGAGAGTTCTCCTGTGATTTCTGAGCCGGTACTAGTTAACATCCACAAGAACGTAGCTATCAATGGGAAGGCTGCAGATGCCAGTGATAGGGCACGGCAACAAGATCCGCAGAGCCAAAGCGTTGTTGAGTCCAGATCATCGGCTCCAGCTGCTCAGCTGGGAGAAGATCAAGCTGCAGCGAAGGCACAGGCAGCCTTTCGTGGTTACCTGGCACGAAGGTCATTCCGTGCATTAAAAGGTATCGTAAGACTCCAGGCGCTGATTCGAGGGTATCTTGTAAGGAGGCAGGCTGTATCAACCCTTCGCGCAACATGGTTGATTGTGAAGTTTCAGGCTCTAGTTCGTGGAAGAAATGTTAGACTCTCTGGCAGTCGCATGCAGCTCAATGTGAAGTTTGGTCAGAGTAACTTTGGGGGTGTTAGATCGTCTGATGCATGGAAAGAGAAGCTATCTTCAAATGCTTATGTTCGGAAGCTTCTGTCTTCACCAATTGTTTTAGAACCTCTTCACTTCCAGTATGACAAGAGGGATCCCAATTCAACCTATAACTGGTTTGAGAGATGGACCATAGGTTGCATCTGGAAGCCTGCTTTTCAACCCAAAAGAGTTGCTGATGGGAAACCACTGGTAAAGAAGGCTAGTTATGCAATGGAAACTCAATCAGCCAAGTTAAAACGCAACATTCGGAAGGGTTCTGCTGCTATCGCTGGGAGTTTCCATACATCTGGTGAATCTGATAAAGTAAAAAGGAATCCAAAGAATTTCTCTAGCTTCCCTGCTGATTCAGTACCAGATAGCCAGTTATCTGAACTTGAAAAGGTTAAAAGGAACCTCAGGAAGGTAACTGATTCGATGGCTGAAGCCTCAAAGATATCTAGTTCCAGGGTTGATTCCTCGAAGGTATGTAATTCTACAGCTGAGGTTCCAAAGGAATCTAATCCTGTGGCAGAAATCTCAAAGATACGTAGTCTCCTGAATGGGATCTCTGACCATCAGGATATTCAATGTGAGAATACACGTGAATCTTCATTTCCTCTTGGAACTCAAGAAGACTCTGACAATGATCATCTATTGCGATATTCAAATATGGATAGCTTGGACTTGGTACCTGGTTTGAAAAGTGATCAGGAAATTCAGCTGGATTCGGTTTCTATAGGAGAAAATGTTGATGATCCCACTGTTGTTGCTCCAGCAGTTGAAGAAATGTCACCGCAAAACATTGATACGGAAGACAATGTTTTATGCAAGAAAGAGGAAGCAAGGTCCAAGGAAGAGCACTTGTCTAATGGAAGCCTTAGAACTAGCAAGAGGAAGTCTTCATTCCCCAACAAATCAGAATATGTAGAAAATGGGACTCACGCTACTCCTGTTCAGCCAAGGCAGCCAAGCTATATGGCTGCAACGGAGTCCGCAAAGGCGAAATTGCGAGCCCAGAATTCACCCAGTCTGGATTCTGATTCAGCGGCAGAAAAGAATGGTTTCACCCGACGCCACTCTCTTCCTTCCAGTACAAAGAGTAGAGCACTTAAAGCTGAATGGAAGCGC
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