| TFome pUT6605 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728324 |
| Protein Name | ZmOrphan220 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G144109 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C236T p.S79F Not present in functional domains. |
| Request Information | Deposited to ABRC |
TFome clone pUT6605
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0374_F |
| Temperature (ºC) | 69.2 |
| Sequence
|
caccATGGGAAACTCCCTCGCCT |
3' Primer
| Name | CD0374_R |
| Temperature (ºC) | |
| Sequence
|
CACGCATGATCTCCAGATCCTG |
PCR Condition
PCR reaction: 25 uL vol 5xGC buffer 5 uL 10 mM dNT
Amino Acid Sequence
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MGNSLACFCCGGGAAGRRRHVAPAALPSDPAYDEGLGHSFCYVRPDKVLVPFSADDDDLVADAKAAAAAEEPTTFRAIFGAALSANVSTQLSTSVLLLLPDESSSAAAASSSSSGFESSESFAAVPLQPVPRFPSGPICAHGGGFLSGPIERGFLSGPLDAALMSGPLPCAATSARMGGGAVPALRRSLSHGGRRLRNFTRALLARADKFQDSMDLGSAAAAACGAGSAGLQWAQGKAGEDRVHIVVSEERGWVFVGIYDGFNGPDATDFLVSHLYAAVHRELRGLLWDQCEQEEQRDTDPDQPMSTTASYHQDQPANGRRARRSRPPRGADDDQRRWKCEWERDCSSLKPPTQRPPRSSSENDHLAVLKALARALRKTEEAYLDVADKMVGEFPELALMGSCVLAMLMKGEDMYLMNVGDSRAVLGTMDSVDLEEISGGSFDGLVGDGTPLLSALQLTSEHSTSVREEVCRIRNEHPGDPSAISKDRVKGSLKVTRAFGAGFLKQPKWNDALLEVFRIDYVGSSPYITCNPSLYHHRLSTRDRFLILSSDGLYQYFTNEEAVAQVEMFIATTPEGDPAQHLVEEVLFRAANKAGMDFHELIEIPHGDRRRYHDDVSVIVISLEGRIWRSCV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGAAACTCCCTCGCCTGCTTCTGCTGCGGGGGCGGCGCCGCCGGCCGCCGCCGCCACGTGGCGCCCGCGGCGCTCCCCTCGGACCCCGCCTACGACGAGGGCCTCGGCCACTCCTTCTGCTACGTCCGGCCGGATAAGGTGCTGGTGCCCTTCTCCGCGGACGACGACGACCTGGTCGCCGACGCCAAGGCGGCCGCCGCGGCCGAGGAGCCCACCACGTTCCGGGCCATATTCGGGGCCGCGCTCAGCGCCAACGTCTCCACGCAGCTCTCCACCTCCGTCCTCCTCCTGCTGCCGGACGAATCCTCCTCCGCCGCCGCCGCCTCCTCCTCCTCCTCCGGGTTCGAGAGCTCCGAATCGTTCGCCGCCGTGCCGCTGCAGCCCGTCCCGAGGTTCCCGTCGGGCCCCATCTGCGCGCACGGGGGCGGGTTCCTCTCCGGGCCCATAGAGAGGGGGTTCCTCTCGGGCCCCCTCGACGCGGCGCTCATGTCCGGCCCTCTCCCTTGCGCCGCCACATCCGCCCGCATGGGAGGAGGCGCCGTGCCCGCGCTCCGCCGGAGTCTCTCGCACGGCGGCCGCCGCCTGCGGAATTTTACCCGCGCGCTTCTCGCGCGGGCGGATAAGTTTCAGGATTCGATGGATCTCGGCTCGGCCGCCGCCGCCGCCTGTGGCGCCGGTTCCGCCGGGCTGCAGTGGGCGCAGGGGAAGGCCGGCGAGGACCGCGTCCACATCGTGGTGTCCGAGGAGCGGGGCTGGGTGTTCGTCGGCATCTACGACGGCTTCAACGGTCCCGACGCCACGGACTTCCTCGTCTCCCATCTCTACGCCGCCGTGCACCGCGAGCTCCGCGGCCTGCTCTGGGATCAGTGCGAGCAGGAGGAGCAGCGCGACACAGATCCCGACCAGCCGATGAGCACCACGGCCTCATATCACCAGGACCAGCCCGCCAACGGCCGCCGCGCCCGCCGATCAAGACCGCCGCGCGGCGCCGATGATGACCAACGGCGGTGGAAGTGCGAGTGGGAGCGCGACTGCTCCAGCCTCAAGCCGCCAACTCAGCGCCCTCCTAGGAGCAGCAGCGAGAACGACCACCTCGCCGTGCTTAAGGCACTTGCGCGCGCGCTTCGCAAGACCGAGGAGGCCTACCTGGACGTCGCCGACAAGATGGTCGGCGAGTTCCCGGAGCTTGCGCTTATGGGCTCTTGCGTTCTTGCCATGCTTATGAAAGGAGAGGACATGTACCTCATGAATGTAGGTGACAGCCGGGCTGTGCTGGGAACAATGGACAGTGTTGATCTCGAGGAGATCAGTGGGGGTTCATTTGATGGATTGGTCGGGGATGGCACTCCGCTCTTGTCAGCTCTGCAGCTCACCTCAGAACATAGCACGTCAGTGCGAGAGGAAGTTTGCAGAATACGGAACGAGCATCCCGGAGACCCGTCTGCAATCTCCAAGGACCGCGTGAAGGGCTCGCTTAAGGTGACGAGAGCGTTTGGAGCTGGTTTCTTGAAACAGCCCAAATGGAACGATGCGCTGCTGGAGGTGTTCAGAATAGATTACGTCGGGTCATCCCCGTACATCACGTGCAATCCTTCGCTCTATCACCACAGGCTCAGCACGAGGGACAGGTTCCTGATACTATCGTCAGACGGGCTCTACCAGTATTTCACCAACGAGGAGGCGGTCGCTCAGGTTGAGATGTTCATCGCAACAACCCCTGAGGGCGACCCTGCCCAGCATCTGGTGGAGGAAGTTCTTTTCAGAGCTGCAAACAAAGCAGGAATGGACTTCCACGAATTGATCGAGATACCACATGGCGACCGCCGGCGGTACCACGACGACGTATCTGTCATTGTGATATCTTTGGAGGGCAGGATCTGGAGATCATGCGTG
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