| TFome pUT6631 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728349 |
| Protein Name | ZmOrphan84 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G420801 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6631
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0400_F |
| Temperature (ºC) | 68.8 |
| Sequence
|
caccATGGTGGTGGGAACGGC |
3' Primer
| Name | CD0400_R |
| Temperature (ºC) | |
| Sequence
|
ATTGTTCTGAAGGACTCTATACAGT |
PCR Condition
5xGC buffer 5 uL 10 mM dNTP 1 uL 100% DMSO 1.25 uL
Amino Acid Sequence
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MVVGTALLRGVSSAWILLFLSSLLLSPSAASVDFGHCGGCDDADDGALSSTYNILQCQKVSDFLIAAAYFSIPLELLYFATCSDLFPLKWIVLQFGAFIVLCGLTHLITVFTYEPHSFHLVLALTVAKFLTALVSFATAITLLTLIPQLLRVKVRENFLMNKARELDREVGRMKRKEEASWHVRMLTQEIRKSLDRHTILYTTMVELSKALELQNCAVWMPDETRSTMILTHQLRERDIMDPQKHSIPIDDPDVQEIKATKDAKVLGPDSALGVSSRSKHEAGPVAAIRMPMLRVSNFKGGTPEVMQTSYAILVLVLPNDGSLGWGRRELEIVEVVADQVAVALSHAALLEESQLMREKLAEQHRDLLQAKDEAMRAGDARNSFQTAMYDGMRRPMHSILGLVSMMQQESMNPEQRLVMDAIAKTSSVASTLMNDVMQTSTMNCEHLSLVRRPFNLHSFIKEVVGVVRCLTGCKGVEFEFQVENSLPERIIGDEKRVFHIVLHMVGTLTDRCNAGCISLYVNVHNEVEDRHNHDWMLRRANFSGGYVCVKFEIRIRKSKGYLLSSSSSQISQGSKPNNSEMGLSFNMCKKIVQMMNGNIWSVSDSKSIGETIMLVLQFQLEPVTPVSGASSDLYRSSAIPNFNGLRVLLADSDCTNRAVTHRLLEKLGCRVLSVASGVQCISSFAAESSFQLVVLDLDMQTMDGFEVARAIRKFSSNSWLPLIIALAARIDDNIRDRCQRSGVNGLIQKPVTLAALGDELYRVLQNN
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGTGGTGGGAACGGCACTGCTGCGCGGGGTTTCCTCCGCGTGGATCCTCCTGTTCCTCTCCTCCCTGCTCCTCTCGCCGTCAGCGGCGTCTGTCGATTTCGGCCACTGCGGCGGCTGCGACGACGCCGACGACGGCGCCCTCTCCAGCACCTATAACATCCTGCAATGCCAGAAGGTCAGCGACTTCCTCATCGCCGCGGCCTACTTCTCCATCCCGCTCGAGCTGCTCTACTTCGCCACCTGCTCCGACCTCTTCCCCCTCAAATGGATCGTGCTGCAGTTCGGCGCCTTCATCGTGCTCTGCGGCCTCACGCACCTCATCACTGTGTTCACCTACGAGCCGCACTCCTTCCACCTCGTACTCGCCCTTACCGTCGCCAAGTTCCTGACGGCACTGGTCTCCTTCGCGACGGCCATCACCCTGCTGACGCTGATACCACAGCTCCTGAGGGTGAAGGTCAGGGAAAACTTCCTGATGAACAAGGCGCGTGAGCTGGACCGGGAGGTGGGGAGGATGAAAAGGAAAGAAGAGGCGAGCTGGCATGTGCGCATGCTCACACAGGAGATCCGCAAGTCGCTCGACAGACATACCATCTTGTACACCACCATGGTTGAGCTCTCGAAGGCACTGGAACTGCAGAATTGTGCTGTCTGGATGCCTGATGAGACCAGGAGCACGATGATCTTAACACATCAGCTGAGGGAAAGGGATATAATGGACCCACAGAAACACTCGATTCCTATTGATGATCCGGATGTTCAAGAAATAAAGGCAACCAAGGATGCAAAAGTTCTTGGCCCAGATTCGGCGCTAGGGGTTTCTAGCCGAAGCAAGCATGAAGCAGGGCCTGTGGCTGCAATAAGGATGCCGATGTTAAGGGTGTCAAATTTCAAAGGAGGGACTCCGGAAGTGATGCAGACGAGCTATGCTATCTTGGTTCTGGTTTTGCCTAATGATGGTTCATTAGGGTGGGGTCGAAGAGAGTTGGAGATTGTTGAGGTAGTTGCTGACCAAGTTGCAGTCGCTCTGTCACATGCTGCACTCCTAGAGGAGTCTCAGCTGATGCGAGAGAAGCTTGCCGAGCAGCATAGGGACTTGCTGCAGGCAAAGGATGAAGCCATGAGGGCAGGGGACGCTAGGAATTCCTTCCAGACTGCAATGTACGATGGAATGCGAAGGCCAATGCACTCAATCCTTGGTCTCGTCTCAATGATGCAACAGGAGAGCATGAATCCAGAGCAAAGGCTTGTGATGGATGCCATTGCCAAGACAAGCAGTGTTGCATCCACACTGATGAACGATGTGATGCAAACATCGACAATGAACTGTGAGCACTTGTCTTTGGTCAGGAGGCCGTTCAACCTTCATTCCTTCATTAAAGAAGTTGTTGGAGTGGTCAGATGTCTAACTGGTTGCAAGGGTGTGGAGTTTGAGTTTCAAGTGGAGAATTCTTTGCCAGAAAGGATCATTGGTGATGAGAAGAGAGTCTTCCATATTGTCCTGCACATGGTAGGCACTCTAACAGACCGATGTAATGCTGGCTGTATCTCATTATATGTAAATGTCCATAATGAGGTTGAAGATAGGCATAATCATGACTGGATGCTGCGAAGAGCAAACTTCTCTGGGGGCTATGTATGTGTGAAATTTGAGATTAGGATTAGAAAATCAAAGGGCTATCTGTTGAGTTCATCAAGCAGTCAGATAAGTCAGGGATCCAAACCCAACAATTCTGAGATGGGGCTTAGCTTCAATATGTGCAAGAAGATTGTGCAGATGATGAATGGCAATATTTGGTCAGTATCAGATTCTAAAAGCATCGGAGAAACTATCATGCTAGTCCTCCAGTTCCAGTTGGAACCTGTGACTCCGGTCTCTGGAGCGTCCTCAGATTTGTACAGATCATCCGCAATTCCCAACTTTAATGGGCTCAGAGTCCTCCTTGCGGACAGCGACTGCACCAACCGAGCTGTAACTCACAGGCTCCTAGAGAAGCTTGGTTGCCGAGTCCTTTCGGTCGCTTCTGGCGTCCAATGCATCAGCTCCTTCGCTGCGGAGTCGTCCTTCCAGCTGGTGGTTCTTGATCTTGACATGCAGACGATGGATGGATTCGAAGTAGCCCGCGCGATCAGGAAGTTCAGTAGCAATAGTTGGCTGCCGTTGATTATTGCCCTAGCAGCAAGAATCGACGACAACATCCGGGATCGTTGCCAGAGGTCAGGAGTAAATGGCCTGATCCAGAAACCGGTCACATTAGCCGCGCTGGGAGATGAACTGTATAGAGTCCTTCAGAACAAT
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