TFome clone pUT6635

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	CD0067_F
Temperature (ºC)	70.5
Sequence Copy to clipboard	caccATGATGATGGCCAACGCGAAGC

3' Primer

Name	CD0067_R
Temperature (ºC)
Sequence Copy to clipboard	AGTACGAAGAGGCCCAGAGAGAGC

PCR Condition

PCR Conditions: per reaction (25l): 10 l GC buffer

Amino Acid Sequence

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MMMANAKLHKQALLPPRSPFPTAAPYADRGPIARPQVGATHHRHGHHQRTSSESFIEEQPPSWLDDLLNEPETPAARQSGRAGHRRSSSDSFALFDGGAAGAGAYANGFEGMGRGGGQPAPWGGVQEYYAKLAPIGRAHGRPWEQGMPNSAGFRHGGGLPMPTKDKVGGHHGPPKVSREHDHGMDKRTDDAGHDQKVGAKEGVPPKHAQSEADNKRAKQQYAQRSRVRKLQYIAELESRVQALQSEGVEVSAEMEFLSQQNIMLDLENKALKQRVESLAQEQLIKRFQQEMFEREIGRLRTIYQQQQQQQAPALVRSNSRDLDVQFANLSLKHKDPNTGRDALSGPLRT

TFome clones do not contain stop codon at 3'end.

CDS

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ATGATGATGGCCAACGCGAAGCTCCACAAGCAGGCCCTGCTGCCGCCGCGGAGCCCCTTCCCGACGGCGGCGCCGTACGCCGACCGCGGCCCAATCGCGCGGCCGCAGGTTGGCGCCACGCACCACCGCCACGGCCACCACCAGCGCACGTCGTCCGAGAGCTTCATCGAGGAGCAGCCGCCGTCGTGGCTCGACGACCTGCTCAACGAGCCAGAGACGCCCGCGGCGCGGCAGAGCGGCCGGGCTGGGCACCGCAGGTCGTCCAGTGACTCGTTTGCGTTGTTTGACGGTGGAGCTGCTGGTGCTGGTGCTTACGCCAATGGTTTCGAGGGGATGGGGAGAGGAGGTGGGCAACCTGCGCCGTGGGGCGGTGTGCAGGAGTACTATGCCAAGCTGGCCCCGATTGGGAGGGCACATGGCCGGCCGTGGGAGCAAGGCATGCCGAATTCGGCGGGCTTCAGGCATGGTGGTGGCCTGCCAATGCCCACAAAAGATAAGGTTGGTGGGCATCATGGGCCACCGAAAGTGTCGAGGGAGCATGACCATGGCATGGACAAGAGAACTGATGATGCTGGCCATGATCAAAAGGTTGGAGCCAAGGAGGGTGTGCCGCCGAAGCATGCACAGTCAGAGGCGGACAACAAGCGTGCTAAACAGCAATATGCTCAGAGGTCTCGTGTCCGGAAGCTGCAGTATATTGCAGAGCTTGAGAGTAGAGTCCAGGCATTACAGTCAGAAGGGGTAGAAGTGTCTGCTGAAATGGAGTTCCTTAGCCAGCAGAATATCATGCTAGACCTGGAAAACAAAGCCTTGAAGCAAAGAGTGGAGAGTCTAGCACAGGAACAACTTATTAAGCGTTTTCAACAGGAGATGTTTGAGCGGGAAATTGGTCGTCTCAGAACAATATATCAGCAACAGCAGCAACAACAGGCTCCTGCTCTTGTTCGTAGTAACAGCAGGGACCTTGATGTGCAGTTCGCCAACTTGTCTCTGAAACACAAAGACCCCAACACGGGCCGGGATGCTCTCTCTGGGCCTCTTCGTACT