| TFome pUT6636 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728354 |
| Protein Name | ZmEREB118 |
| Species | Zea mays |
| TF Family | AP2/ERF-ERF |
| Gene id | GRMZM2G081892 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT6636
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0054_F |
| Temperature (ºC) | 73.4 |
| Sequence
|
caccATGGCCCCGTTGAACCACCAGC |
3' Primer
| Name | CD0054_R |
| Temperature (ºC) | |
| Sequence
|
CACGCAGAAATCAACGGTTTGCAG |
PCR Condition
PCR Conditions: per reaction (25l): 10 l GC buffer
Amino Acid Sequence
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MAPLNHQQHVLLKKALAKKPKAKRMSRFGLKPSAAALLKSRPQQPAPAPAQPRRRVRVLFEDPDATDSDSDDEEAGASTCPAKAKRFSFETFVGKVPARPVLPAAAVAACASGGAPESYRGVRLRKWGKWAAEIRNPFTGKRQWLGTFDTAGAASAAYLSASRSFADEKRRRRGQPVPSSSPVPSASATPTASSSSSTSTAPFAHPSPSSVLGPATKEAPLNPQSPELVATPTLVLPSDESAELPDDPEFYQDLLRGLQLPDISPMDFRAGLDALDVSDAPFGLDDEQDLLLGDFGDEELEIDLDLDDINDVFPEMPGCDLGRGMDDFLQTVDFCV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCCCGTTGAACCACCAGCAGCATGTTCTCCTCAAGAAGGCCCTGGCGAAGAAACCCAAAGCTAAGAGGATGTCCAGGTTTGGCCTTAAACCCTCCGCTGCAGCCCTCCTGAAGTCCCGGCCGCAGCAGCCGGCGCCTGCCCCGGCCCAGCCGAGGCGCCGCGTCCGGGTTCTCTTCGAGGATCCTGACGCCACGGACTCGGACTCCGACGACGAGGAGGCCGGCGCCTCTACGTGCCCGGCGAAGGCCAAGCGCTTCTCCTTCGAGACGTTCGTCGGCAAGGTCCCAGCCAGGCCGGTCCTTCCGGCGGCCGCTGTCGCCGCGTGCGCCAGTGGCGGGGCCCCGGAGAGCTACCGCGGAGTGAGGCTCCGCAAGTGGGGCAAGTGGGCGGCGGAGATCCGCAACCCGTTCACGGGCAAGAGGCAGTGGCTTGGCACCTTCGACACGGCCGGCGCGGCCTCCGCAGCCTACCTATCCGCTTCCCGGAGCTTCGCCGACGAGAAGCGCCGCCGCCGCGGGCAGCCTGTGCCCTCTTCCTCACCAGTTCCTTCGGCAAGCGCCACCCCGACGGCCTCCTCTTCGTCGTCCACCTCGACTGCTCCGTTCGCGCACCCGTCGCCGTCGTCTGTTCTTGGACCCGCCACCAAGGAGGCGCCGCTGAACCCGCAGTCCCCGGAGCTGGTCGCGACACCAACGCTCGTCCTGCCGTCCGATGAGTCCGCGGAGCTGCCGGACGACCCGGAGTTCTACCAGGACCTTCTCCGCGGCCTGCAGCTGCCAGACATCAGCCCGATGGACTTCCGTGCCGGGCTTGACGCTCTGGACGTCTCCGATGCGCCCTTCGGCCTGGACGATGAGCAGGACCTGCTGCTTGGGGACTTCGGGGACGAGGAACTGGAGATCGACCTCGACCTCGACGATATCAACGACGTCTTCCCGGAGATGCCTGGCTGCGACCTCGGCCGCGGCATGGACGATTTCCTGCAAACCGTTGATTTCTGCGTG
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