| TFome pUT6646 | |
|---|---|
| Template | BT063335 |
| Genbank Insert ID | KJ728364 |
| Protein Name | ZmOrphan14 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | AC155624.2_FG006 |
| transcript | T006 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.G352A p.A118T; c.T936C p.A312(=) Not present in template FLcDNA. Present in functional domain. |
| Request Information | Deposited to ABRC |
TFome clone pUT6646
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C510_F |
| Temperature (ºC) | 72.2 |
| Sequence
|
caccATGCTGGCCGCGGTGATGG |
3' Primer
| Name | C510_R |
| Temperature (ºC) | |
| Sequence
|
TGGCATGAACACCACGACGA |
PCR Condition
Volumes per reaction (25l): 5 l GC buffer (Thermo
Amino Acid Sequence
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MLAAVMDYFSSCWGPRSGAGHREKGSDAAGRQDGLLWYKDVGQLVTGEFSMAVVQANQLLEDQSQVESGSLSLADTGPQGTFVGVYDGHGGPETSRFINDHLFNHLRRFATEHKFMSTDVIRKAFQATEEGFLSLVSKEWSLKPQIASVGSCCLVGVICAGTLYVANLGDSRAVLGRLVKATGEVLAIQLSSEHNACYEEVRQELQSSHPDDPHIVVLKHNVWRVKGIIQISRSIGDVYLKKPEYNREPLHSKFRLRETFQRPILSSEPQITEHRIQPNDQFVIFASDGLWEHLSNKEAVDLVQSSPRNGIARRLVKAAMQEAAKKREMRYSDLKKIDRGVRRHFHDDITVVVVFMP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCTGGCCGCGGTGATGGACTACTTTAGCTCCTGCTGGGGCCCGCGATCGGGTGCCGGGCACCGGGAAAAGGGCTCCGACGCCGCTGGCCGGCAGGACGGTCTCCTCTGGTACAAGGACGTCGGGCAGCTCGTCACTGGGGAGTTCTCCATGGCCGTGGTGCAGGCCAACCAGCTGCTCGAGGACCAGAGCCAAGTGGAGTCCGGATCGCTCTCCCTGGCTGACACGGGCCCGCAGGGCACCTTCGTCGGCGTCTATGATGGCCATGGCGGCCCGGAGACGTCCCGGTTCATCAATGACCACCTCTTCAACCATCTCAGAAGGTTTGCAACTGAGCACAAGTTTATGTCAACGGACGTGATCCGGAAAGCTTTCCAAGCAACTGAGGAGGGCTTTCTTTCTCTAGTCAGCAAGGAATGGTCTTTGAAGCCTCAGATTGCTTCAGTGGGCTCCTGCTGCCTTGTTGGTGTAATCTGTGCTGGGACTCTCTATGTTGCAAACCTGGGCGACTCACGTGCAGTTCTTGGAAGGCTTGTTAAGGCAACTGGAGAGGTTTTGGCCATTCAGTTGTCATCGGAGCACAATGCGTGCTATGAGGAAGTTAGACAAGAGCTGCAGTCATCACATCCTGATGATCCACATATTGTTGTTCTCAAACACAATGTTTGGCGTGTGAAGGGTATCATCCAGATCTCAAGATCTATTGGAGATGTATATCTAAAGAAACCAGAGTATAATAGAGAACCGCTTCACAGCAAGTTTCGGCTTCGAGAAACCTTCCAGAGGCCGATCCTTAGTTCTGAACCTCAAATTACTGAACATCGAATACAGCCAAACGATCAATTTGTTATATTTGCTTCTGATGGTCTATGGGAGCACCTCAGCAATAAGGAAGCAGTTGACCTTGTCCAAAGTAGTCCCCGAAATGGAATCGCCCGGAGACTAGTGAAAGCCGCGATGCAAGAAGCTGCCAAGAAGAGGGAGATGAGATATTCAGACCTCAAGAAGATCGACCGTGGTGTGAGAAGGCATTTCCACGACGATATAACTGTCGTCGTGGTGTTCATGCCA
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