| TFome pUT6681 | |
|---|---|
| Template | RT-PCR |
| Genbank Insert ID | KJ728397 |
| Protein Name | ZmOrphan134 |
| Species | Zea mays |
| TF Family | Orphans |
| Gene id | GRMZM2G110541 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.T59A p.V20D Present in first Zn functional domain. |
| Request Information | Deposited to ABRC |
TFome clone pUT6681
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | CD0364_F |
| Temperature (ºC) | 68.6 |
| Sequence
|
caccATGAAGGTGCAGTGCGACG |
3' Primer
| Name | CD0364_R |
| Temperature (ºC) | |
| Sequence
|
GGTACTCGGAGATGCTGCTG |
PCR Condition
PCR Reaction Mix:5 l GC buffer (Thermo Fisher) 1 l
Amino Acid Sequence
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MKVQCDVCAAEAASVFCCADEAALCEACDRRVHRANKLAGKHRRLSLLSPAAPSSSAQQTPPPPLCDICQEKRGLLFCKEDRAILCPDCDVPVHTASELAMRHTRFLLTGVRLSGSPQSPPRARRRRRRTRTTAAAAASPST
TFome clones do not contain stop codon at 3'end.
CDS
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ATGAAGGTGCAGTGCGACGTGTGCGCGGCCGAGGCGGCGTCTGTGTTCTGCTGCGCCGACGAGGCCGCGCTGTGCGAGGCGTGCGACCGCCGCGTGCACCGCGCCAACAAGCTCGCCGGCAAGCACCGCCGGCTCTCGCTGCTCAGCCCGGCGGCGCCGTCCTCGTCGGCGCAGCAGACACCGCCGCCTCCGCTCTGCGACATCTGCCAGGAGAAGCGCGGGCTCCTGTTCTGCAAGGAGGACCGGGCGATCCTGTGCCCCGACTGCGACGTGCCGGTGCACACGGCCAGCGAGCTGGCCATGCGCCACACCCGGTTCCTGCTCACGGGCGTGCGCCTCTCCGGCTCTCCGCAGAGCCCGCCGCGCGCCCGCCGCCGTCGGAGGAGGACGAGAACGACAGCAGCAGCAGCAGCATCTCCGAGTACC
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