GrassTFDB :: Browse Family::Sorghum

Displaying C2C2-GATA Family from Sorghum


Many light-responsive promoters contain GATA motifs and a number of nuclear proteins have been defined that interact with these elements. Type-IV zinc-finger proteins have been extensively characterised in animals and fungi and are referred to as GATA factors by virtue of their affinity for promoter elements containing this sequence. We previously identified cDNA sequences representing four Arabidopsis thaliana type-TV zinc-finger proteins. Here we define the organisation and expression of GATA-1 GATA-2 GATA-3 and GATA-4 as well as DNA-binding characteristics of their encoded proteins. Transcripts from all four genes can be detected in all tissues examined suggesting that they are not developmentally regulated at the level of transcription. In vitro binding experiments with Escherichia coli-derived recombinant proteins were performed using motifs previously defined as targets for nuclear GATA-binding proteins. These studies reveal differences in DNA binding specificity of GATA-1 as compared to the other three proteins. In vivo protein-DNA interactions monitored by yeast one-hybrid assays reveal different binding characteristics as compared to those defined with E. coli-derived recombinant protein. Trans-activation of gene expression by the four Arabidopsis proteins via some but not all DNA elements tested indicates that the Arabidopsis proteins can form functional interactions with previously defined promoter elements containing GATA motifs. We conclude that the Arabidopsis type-IV zinc-finger proteins may represent the previously defined family of nuclear GATA-binding proteins implicated in light-responsive transcription.

Protein Name
accepted / suggested
Gene Locus Synonym/
Gene Name
Clone in TFome Genome Browser
SbGATA1 Sb01g002270 View in Browser
SbGATA2 Sb01g005690 View in Browser
SbGATA3 Sb01g011980 View in Browser
SbGATA4 Sb01g020090 View in Browser
SbGATA5 Sb01g029170 View in Browser
SbGATA6 Sb03g013700 View in Browser
SbGATA7 Sb03g030310 View in Browser
SbGATA8 Sb03g034360 View in Browser
SbGATA9 Sb03g047520 View in Browser
SbGATA10 Sb04g002090 View in Browser
SbGATA11 Sb04g002093 View in Browser
SbGATA12 Sb04g007750 View in Browser
SbGATA13 Sb04g033450 View in Browser
SbGATA14 Sb04g036520 View in Browser
SbGATA15 Sb05g001890 View in Browser
SbGATA16 Sb05g022380 View in Browser
SbGATA17 Sb06g023940 View in Browser
SbGATA18 Sb06g024200 View in Browser
SbGATA19 Sb08g004340 View in Browser
SbGATA20 Sb08g004360 View in Browser
SbGATA21 Sb08g004370 View in Browser
SbGATA22 Sb08g022276 View in Browser
SbGATA23 Sb09g004315 View in Browser
SbGATA24 Sb09g025950 View in Browser
SbGATA25 Sb09g028850 View in Browser
SbGATA26 Sb09g029510 View in Browser
SbGATA27 Sb10g022580 View in Browser