TFome clone pUT1146

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	C43 UP
Temperature (ºC)	62.0
Sequence Copy to clipboard	CAC CAT GGA CTG GGC AGC G

3' Primer

Name	C43 DN
Temperature (ºC)
Sequence Copy to clipboard	CTGCCAAGAGAATGGCAGtgc

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MDWAAASGSPSWGAAATDPGPTMLSFAGPSSSAADAEVRLQDFAAGLAQSSRPTGAAPGAGRRSRAAVGGAGAEACSVDGCRSDLSRCREYHRRHKVCEAHSKAPVVVVGGQERRFCQQCSRFHMLSEFDEEKRSCRKRLDGHNRRRRKPQHDLTNLGGFFPYHQVNQFEFYPQTIPTVGQNSDAVHLVGRQQPFSISFSRTPDEFPFPQGGGSSVLHHLHAARPGHHAEGSSHTGSSTRDNALSGTLGPECALSLLSSSLRRPSTAGVPAVGQPQVTASSTTAVSQAATAAVATAAFAASAGHHVFVPDAMFEDPSQALPFSWQ

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACTGGGCAGCGGCGTCCGGGTCTCCGTCGTGGGGCGCGGCGGCGACGGACCCCGGTCCGACCATGCTGTCCTTCGCAGGCCCGTCCTCCTCCGCCGCCGACGCCGAGGTGCGGCTCCAGGATTTCGCCGCGGGTCTTGCCCAGAGCTCGCGGCCGACTGGGGCGGCGCCGGGAGCCGGGAGGAGGTCCCGCGCGGCCGTGGGAGGTGCCGGCGCGGAGGCGTGCTCCGTCGACGGCTGCCGCTCTGATCTCAGCCGGTGCCGCGAGTACCACCGGCGGCATAAGGTCTGTGAGGCACACTCCAAGGCGCCGGTGGTGGTCGTCGGCGGACAGGAGCGGCGCTTCTGCCAGCAATGCAGCCGGTTTCACATGCTGTCCGAGTTTGACGAGGAAAAAAGAAGTTGCCGGAAGCGTCTGGATGGCCATAATAGGCGTCGAAGAAAGCCACAGCATGATCTGACAAATCTAGGGGGTTTCTTTCCATACCACCAAGTGAATCAGTTTGAGTTCTACCCACAAACAATTCCAACAGTTGGCCAGAATTCGGACGCCGTGCACTTGGTGGGCCGTCAGCAGCCTTTCTCCATATCGTTCTCAAGAACACCAGACGAGTTCCCTTTCCCACAGGGTGGCGGTAGCAGTGTGCTGCACCACCTCCATGCAGCCCGCCCTGGCCATCACGCTGAAGGCAGCAGCCACACTGGAAGCAGCACACGCGACAACGCTCTCTCCGGCACACTGGGCCCCGAGTGTGCTCTCTCTCTTCTGTCATCGTCGCTGCGTCGCCCCTCAACTGCTGGCGTCCCCGCCGTTGGCCAACCCCAGGTTACTGCCTCTTCGACCACTGCCGTGTCGCAGGCTGCAACGGCAGCTGTGGCCACTGCTGCATTCGCGGCCAGTGCTGGCCACCATGTGTTTGTTCCTGATGCTATGTTTGAGGATCCATCACAGGCACTGCCATTCTCTTGGCAG