| TFome pUT1149 | |
|---|---|
| Template | AM156907 |
| Genbank Insert ID | HQ858693 |
| Protein Name | ZmMYB39 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G127857 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | c.G6C p.(=); PZE04141737314 c.C80T p.A27V; c.C352G p.P118A; PZE04141737942 c.A355C p.K119Q; PZE04141737945 c.A453G p.(=); PZE04141738043 c.G469T p.A157S; c.G501A p.(=); PZE04141738091 c.C529G p.P177A; PZE04141738119 c.G554A p.G185D; PZE04141738144 c.C567A p.(=); PZE04141738157 c.C621G p.(=); PZE04141738211 c.A682G c.A683C p.K228A; c.408del12. Clone is from inbred line W64, obtained from David Capparos. Present in template FlcDNA. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT1149
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | D3 UP |
| Temperature (ºC) | 61.6 |
| Sequence
|
CAC CAT GGG CCG CTC TCC |
3' Primer
| Name | D3 DN |
| Temperature (ºC) | |
| Sequence
|
AATAATCTGGGGCAAATTctgc |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MGRSPCCEKMGLKRGPWTAEEDRILVVHVERHGHSNWRALPKQAGLLRCGKSCRLRWINYLRPDIKRGNFSREEEDAIIQLHQMLGNRWSTIAARLPGRTDNEIKNVWHTHLKKRLEAQPASQQAPKRKPTKKQQPEPVTTLEGPAGAVPPVSPERSLSTTTSTTTSTADYSAASSLENADDSFTSEEDYYQIDDSFWSETLAMTTTVDSFESGVQQAEGSFGASAAAPSSTNDDMDFWLKLFMQASDMQNLPQII
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGCCGCTCTCCGTGCTGCGAGAAGATGGGGCTGAAGAGGGGTCCGTGGACGGCCGAGGAGGACAGGATTCTGGTGGTGCACGTCGAGCGCCACGGCCACAGCAACTGGCGCGCGCTGCCCAAGCAGGCGGGCCTGCTCCGCTGCGGCAAGAGCTGCCGCCTCCGCTGGATCAACTACCTGCGCCCCGACATCAAGCGCGGCAACTTCAGCCGGGAGGAGGAGGACGCCATCATACAGCTCCACCAAATGCTCGGCAACAGATGGTCGACAATCGCTGCCAGGCTGCCGGGGAGGACGGACAACGAGATCAAGAACGTCTGGCACACCCACCTCAAGAAGCGCCTGGAGGCACAGCCAGCCAGCCAGCAGGCGCCCAAGCGCAAGCCCACGAAGAAGCAGCAGCCCGAGCCCGTAACGACGCTCGAGGGGCCGGCCGGGGCCGTGCCGCCGGTGTCGCCGGAGCGGTCACTCTCCACGACGACGTCAACCACCACCAGCACCGCGGACTACTCGGCCGCCTCGTCGCTGGAGAACGCGGACGACAGCTTCACATCGGAGGAGGACTACTACCAGATCGACGACAGCTTCTGGTCCGAGACGCTGGCGATGACGACGACGGTGGACAGCTTCGAGTCCGGGGTGCAGCAGGCGGAGGGCAGCTTCGGCGCGTCGGCGGCGGCGCCGTCGTCGACCAACGACGACATGGACTTCTGGCTCAAGCTGTTCATGCAGGCCAGCGACATGCAGAATTTGCCCCAGATTATT
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