| TFome pUT1157 | |
|---|---|
| Template | DR785526 |
| Genbank Insert ID | HQ858701 |
| Protein Name | ZmMYB50 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G145444 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT1157
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B33 UP |
| Temperature (ºC) | 61.4 |
| Sequence
|
caccATGGGAGTGGAGTCGGAG |
3' Primer
| Name | B33 DN |
| Temperature (ºC) | |
| Sequence
|
CGGCCGTGTGACGACGCC |
PCR Condition
Step 1, 95C 4 min melt, 1 cycle; Step 2, 95C 30 se
Amino Acid Sequence
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MGVESECDRIKGPWSPEEDEALRRLVERHGARNWTAIGRGIPGRSGKSCRLRWCNQLSPQVERRPFTPEEDAAILAAHARLGNRWAAIARLLPGRTDNAVKNHWNSSLKRKLATATATSSGSDAERACKRASPGPGSPTASDRSDLSHGCGAAQVFRPVPRAGGFDAISAADVRPPPPPAVADEDPLTSLSLSLPGLDQSPSGFHHDSARSHFQELSPSSSPSPQSTPPAFPPQAAPSQSSYAFSGELVAAMQEMIRAEVRKYMSGAGLRAGCGPGAVGEVCMPQLVEGVMRAAAERVGVVTRP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGAGTGGAGTCGGAGTGCGACAGGATAAAGGGCCCCTGGAGCCCCGAGGAGGACGAGGCGCTGCGGCGTCTCGTGGAGCGCCACGGCGCGCGGAACTGGACGGCGATCGGGCGCGGCATCCCGGGGCGGTCGGGGAAGTCGTGCCGGCTGCGGTGGTGCAACCAGCTGTCGCCGCAGGTGGAGCGCCGCCCCTTCACGCCCGAGGAGGACGCCGCGATCCTGGCCGCGCACGCCCGGCTGGGCAACCGGTGGGCCGCCATCGCGCGCCTTCTCCCGGGGCGCACCGACAACGCCGTTAAGAACCACTGGAACTCGTCGCTCAAGCGGAAGCTCGCCACCGCCACGGCGACCTCCAGCGGCAGCGATGCTGAGCGGGCGTGCAAGCGCGCAAGCCCTGGCCCGGGGAGCCCGACGGCGTCGGACCGCAGCGACCTCAGCCACGGCTGCGGCGCCGCGCAGGTGTTCCGTCCCGTCCCGCGCGCCGGAGGCTTCGACGCCATCAGCGCCGCAGACGTGCGCCCTCCGCCCCCGCCAGCAGTCGCAGACGAGGACCCGCTCACCTCGCTCTCCCTCTCGCTGCCGGGGCTGGACCAGTCGCCGTCGGGCTTCCACCACGACAGCGCCCGGAGCCACTTCCAGGAGCTCTCCCCCTCGTCGTCCCCGTCGCCGCAGTCTACTCCACCCGCCTTCCCTCCGCAGGCGGCTCCGTCTCAGTCGTCGTACGCGTTCAGCGGCGAGCTGGTTGCCGCGATGCAGGAAATGATCCGCGCCGAGGTGCGCAAGTACATGTCCGGCGCGGGGCTCCGCGCCGGATGCGGGCCCGGGGCCGTGGGCGAGGTGTGCATGCCGCAGCTGGTGGAGGGTGTGATGCGCGCCGCCGCGGAGCGCGTCGGCGTCGTCACACGGCCG
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