| TFome pUT1159 | |
|---|---|
| Template | |
| Genbank Insert ID | HQ858703 |
| Protein Name | ZmIAA7 |
| Species | Zea mays |
| TF Family | Aux/IAA |
| Gene id | GRMZM2G138268 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1159
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B71 UP |
| Temperature (ºC) | 61.0 |
| Sequence
|
caccATGGCGACAACGACGG |
3' Primer
| Name | B71 DN |
| Temperature (ºC) | |
| Sequence
|
GCTTCTGTTCTTGCATTTctc |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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matttdlgfeatelrlglpggsggggggelalggegrssssasgkrgfaetidlklklepaavveaeeeeedhgvavalekeeeagkmkrspsqssvaaaavladpaekpraakaqvvgwppvrsfrknimsvqsdkgagkdaaaangdkssaaagggaafvkvsldgapylrkvdlkmyrsyqqlskalenmfssftigscgsqgmngmnesklvdllngseyvptyedkdgdwmlvgdvpwemfvesckrlrimkgseaiglapramekcknrs
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGACAACGACGGACCTGGGGTTCGAGGCGACGGAGCTCCGCCTGGGCCTGCCCGGCGGCAGCGGCGGGGGAGGGGGAGGGGAGCTGGCGCTGGGCGGCGAGGGGAGGAGCTCGTCCTCCGCCTCCGGCAAGAGGGGCTTCGCCGAGACCATCGACCTGAAGCTGAAGCTGGAGCCAGCGGCCGTCGTGGAGGCGGAGGAGGAGGAGGAGGACCACGGCGTTGCTGTTGCCCTTGAGAAGGAGGAGGAGGCCGGGAAGATGAAGCGGTCCCCGAGCCAGAGCAGCGTCGCCGCCGCCGCCGTGCTGGCTGACCCCGCCGAGAAGCCGCGCGCCGCCAAGGCTCAGGTGGTGGGATGGCCACCGGTCCGGTCGTTCCGGAAGAACATCATGTCCGTGCAGTCCGACAAGGGCGCCGGCAAAGACGCCGCCGCCGCCAACGGCGACAAGTCCTCGGCGGCGGCAGGCGGCGGTGCCGCGTTCGTGAAGGTGAGCTTGGACGGCGCGCCCTACCTGCGCAAAGTGGACCTCAAGATGTACAGGAGCTACCAGCAGCTGTCCAAGGCGCTCGAGAACATGTTCAGCTCCTTCACCATCGGAAGCTGTGGGTCTCAAGGGATGAACGGCATGAACGAGAGCAAGCTGGTGGATCTGCTCAACGGCTCCGAGTACGTGCCGACCTACGAGGACAAGGACGGGGACTGGATGCTCGTCGGCGATGTGCCGTGGGAGATGTTCGTCGAATCATGCAAGCGCCTTCGGATCATGAAAGGATCAGAAGCCATTGGCCTGGCACCAAGGGCCATGGAGAAATGCAAGAACAGAAGC
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