| PCR Condition:
|
|
| Nucleotide Sequence:
|
ATGGCGGGGAGTGGGAGGCCGCCGGCGGCGCAGAAGAAGATCCTGCAGTCGCTGCGCCCGCCGCTGCCGTTCGCCGCCTCGTCGAGGTCGCCCTTCGCGGCGCCCAACGACTACCACCGCTTCCCCGCTGGTGGCGCGGCGGCGGCGGCGGCGTCTGGTTCGGGTGGCATCGGCGCGGGCGGTGCTGGCGGCGGCGGCGATATCGAGGAGGGGCTGGTCATCCGGACGCCGCAAAAAAGAAAAGCACCAGAAGAAAGTGATGTTGCTGAGTCTAGCGACTGTATGATTACTAGCCCTGGATTTGCTGTCAGTCCAATGCTCACTCCAGTCTCTGGGAAAGCTGTTAAGACTTCCAAGTCAAAGACTAAGAACAACAAAGCTGGGCCTCAGACACCTACATCAAATGTTGGTTCACCACTTAATCCCCCAACTCCTGTTGGTACATGCCGTTATGACAGTTCGTTAGGGCTTCTCACAAAGAAGTTCATAAATTTGCTGAAGCAGGCTCCGGATGGCATTCTAGATTTGAATAATGCTGCAGAAACACTAGAGGTTCAAAAACGGCGTATATATGATATTACCAATGTACTTGAAGGAATAGGGTTGATTGAAAAGACACTCAAGAACAGAATCCGTTGGAAGGGCTTGGATGATTCAGGAGTGGAATTAGATAATGGTCTTTCAGCTTTGCAGGCAGAAGTTGAAAATCTTAGTCTGAAGGAGCAAGCATTAGATGAGCGCATAAGTGATATGCGTGAAAAACTAAGAGGTTTAACTGAAGATGAAAACAATCAAAGATGGCTCTATGTGACAGAAGATGATATCAAGGGATTACCCTGCTTTCAGAATGAAACACTAATTGCAATAAAAGCTCCTCATGGCACTACACTTGAAGTCCCAGATCCTGACGAGGCTGGTGATTATCTCCAGAGAAGATATAGAATCGTATTAAGAAGTACAATGGGCCCAATAGATGTCTACTTAGTTAGTCAATTTGATGAGAAATTTGAGGACTTGGGTGGGGGTGCAACACCATCAGGGCATGCAAATGTACCAAAACATCAACCTACTGAAGTCTTCAATACAACAAATGCTGGGGTTGGGCAATGTAGCAACTCAGTTGCTGTGGACAATAATATTCAGCACAGCCAGACGATTCCTCAGGATCCTAGTGCTTCACATGATTTTGGAGGAATGACAAGGATTATCCCTTCAGATATTGATACTGATGCTGATTACTGGCTCATATCAGAAGGGGATGTCAGCATTACTGATATGTGGAAAACAGCACCAGATGTGCAGTGGGATGAGAGCCTGGATACGGATGTCTTCCTATCTGAAGACGTTAGAACGCCAAGTTCACACAATCAGCAGCCATCTGCAGTGGGCGGGCCACAGATGCAGGTCTCAGACATGCATAAACCC |
|
|
TFome clones do not contain stop codon at 3'end.
|
| translation:
|
magsgrppaaqkkilqslrpplpfaassrspfaapndyhrfpaggaaaaaasgsggigaggaggggdieeglvirtpqkrkapeesdvaessdcmitspgfavspmltpvsgkavktsksktknnkagpqtptsnvgsplnpptpvgtcrydsslglltkkfinllkqapdgildlnnaaetlevqkrriyditnvlegigliektlknrirwkglddsgveldnglsalqaevenlslkeqalderisdmreklrgltedennqrwlyvteddikglpcfqnetliaikaphgttlevpdpdeagdylqrryrivlrstmgpidvylvsqfdekfedlgggatpsghanvpkhqptevfnttnagvgqcsnsvavdnniqhsqtipqdpsashdfggmtriipsdidtdadywlisegdvsitdmwktapdvqwdesldtdvflsedvrtpsshnqqpsavggpqmqvsdmhkp
|
