| TFome pUT1394 | |
|---|---|
| Template | DR962345 |
| Genbank Insert ID | HQ858726 |
| Protein Name | ZmNLP1 |
| Species | Zea mays |
| TF Family | NLP |
| Gene id | AC233880.1_FG006 |
| transcript | T006 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1394
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B89 UP |
| Temperature (ºC) | 67.3 |
| Sequence
|
caccATGGCTTCCGACGCCCCG |
3' Primer
| Name | B89 DN |
| Temperature (ºC) | |
| Sequence
|
GAC ACG AGT AAT ACA ACA TTC TGG CAG |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MASDAPAEQPATQQKPTRVSLSYEEISKLFSLPIAEAASILGVCTSVLKRICRTHGIVRWPYRKLVSGKAGDDTKGPDSDKANELLEVSKIAKQKAPSASGPSVVSSSTSQGAAKSQQGNSKAGQFSVSPPTGKHNASLSLTHSQAKAIPCYMDDFKYGFPSSGLSCETMKWWGTSSDTDYVPTKDGSHEPHESTTHEPSKGMTDDDELDWGADEAEAEADGTVTAEASAQLCSLRRKAVDDGRKLLNGHNRRGQEFSRLNKRQKTALAQVFGASLPECCITRV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCTTCCGACGCCCCGGCGGAGCAACCGGCGACGCAGCAGAAGCCCACCAGGGTCTCGCTGTCCTATGAGGAGATCTCCAAGCTCTTCTCCCTCCCCATCGCTGAGGCGGCCTCCATCCTCGGAGTCTGCACCAGTGTCTTGAAGAGGATCTGCCGCACCCACGGGATTGTTAGGTGGCCATATCGTAAGCTTGTTTCTGGGAAAGCTGGGGATGACACAAAAGGTCCTGATAGCGACAAAGCCAACGAACTCCTTGAGGTATCAAAAATCGCGAAACAAAAGGCTCCCAGTGCATCAGGCCCATCAGTAGTGTCATCAAGCACTTCCCAAGGAGCGGCAAAGTCTCAACAGGGCAATTCTAAGGCAGGACAGTTTTCAGTTTCGCCACCAACAGGTAAACATAACGCATCACTAAGTTTGACCCATAGCCAAGCCAAGGCCATCCCTTGCTATATGGATGATTTCAAGTACGGATTCCCGTCATCTGGCTTGTCTTGTGAAACTATGAAATGGTGGGGCACAAGCAGCGACACAGACTATGTGCCTACCAAAGATGGAAGCCATGAACCCCATGAATCAACAACACATGAGCCATCAAAGGGCATGACTGATGATGACGAGTTGGACTGGGGAGCAGATGAAGCGGAAGCTGAAGCTGATGGCACTGTTACGGCAGAGGCATCGGCACAGCTGTGCTCGCTGAGAAGGAAAGCAGTAGATGACGGGCGCAAACTATTGAATGGTCACAACCGCAGGGGCCAAGAATTCTCTAGGCTGAACAAAAGGCAGAAGACAGCGCTAGCCCAGGTATTTGGGGCTTCACTGCCAGAATGTTGTATTACTCGTGTC
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