TFome clone pUT1397

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B102 UP
Temperature (ºC)	63.7
Sequence Copy to clipboard	CAC CAT GCC TCG GGA TGG CG

3' Primer

Name	B102 DN
Temperature (ºC)
Sequence Copy to clipboard	TTCACAGGCTAGCTTGGTC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

Copy to clipboard

MPRDGAQAAPPPERGEMDEVVEADPDTEAEDQEERWARLLPELLSDVVRRVEASGGERWPARKDVVSCACVCRRWREAAVSVVRPPAESGKITFPSSLKQPGPRERPMQCFIKRNKNNSTFYLYLGFTSSPVDKGKFLMAARRFRRGPHTEYIISLDAEDLSQGSNAYMGKLRSDFWGTNFKIYDSKPPYDGAKASSSRSSRRFGSRRISPQVSAGNYEVGQVSYKYNLLKSRGPRRMYCTLECPSAQETWENSLKTKFRRPLGPTTLRNKAPRWHEHLQCWCLNFHGRVTVASVKNFQLVAAADPSEPTTNVDDETVLLQFGKVDSDMFTMDYRQPLSAFQAFAISLSSFGTKLACE

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGCCTCGGGATGGCGCGCAGGCGGCGCCGCCACCGGAGCGCGGCGAGATGGACGAGGTGGTAGAGGCGGACCCGGATACGGAGGCGGAGGATCAGGAGGAGAGGTGGGCGAGGCTGCTGCCGGAGCTGCTCTCCGACGTGGTGCGGCGCGTCGAGGCGTCCGGCGGCGAGCGGTGGCCGGCGCGGAAGGACGTCGTCTCCTGCGCCTGCGTGTGCCGCCGGTGGCGGGAGGCCGCCGTCTCCGTCGTGCGTCCGCCGGCGGAGTCCGGCAAGATCACCTTCCCCTCCTCGCTCAAGCAGCCAGGGCCAAGGGAGAGACCAATGCAGTGCTTTATCAAGAGGAATAAGAACAACTCTACATTCTACCTCTACCTTGGCTTCACAAGTTCACCTGTGGATAAAGGGAAGTTTCTCATGGCTGCTAGAAGATTTAGGCGTGGTCCCCATACCGAGTACATTATATCTCTTGATGCAGAAGACTTATCACAAGGGAGCAATGCATACATGGGGAAACTGAGATCTGATTTCTGGGGGACAAACTTCAAAATATATGATAGCAAGCCACCATATGATGGTGCTAAAGCATCAAGTAGTCGATCTAGTCGTCGTTTCGGAAGCAGAAGGATTAGTCCCCAGGTATCGGCTGGCAATTATGAAGTTGGACAGGTTTCATACAAATACAACTTGCTCAAATCCAGAGGCCCCAGGAGAATGTATTGCACTCTCGAATGCCCTTCAGCCCAAGAGACTTGGGAAAACTCACTCAAGACAAAGTTCAGGCGCCCCCTGGGCCCCACAACTTTACGAAATAAAGCACCCCGCTGGCATGAGCACCTGCAATGCTGGTGCTTAAACTTCCATGGGCGGGTAACAGTTGCATCAGTCAAGAATTTCCAGCTTGTGGCTGCTGCCGACCCCAGTGAGCCTACTACCAACGTGGATGATGAAACGGTTCTGCTGCAGTTTGGTAAGGTCGATAGCGATATGTTCACAATGGATTACCGGCAACCACTCTCCGCGTTTCAAGCATTTGCCATCAGCCTCAGCAGCTTTGGGACCAAGCTAGCCTGTGAA