TFome clone pUT1453

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B88UP
Temperature (ºC)	70.0
Sequence Copy to clipboard	CAC CAT GGC GGG TGG CGG CGA G

3' Primer

Name	B88DN
Temperature (ºC)
Sequence Copy to clipboard	CTC TGG AGA AGA AAC CAT ATC TAC CTC CCT GGA CGA TG

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MAGGGEGDRVGVGGAQQFVDRSKVRILLCDGDATSSREVLRLLCNCSYHVTCAKSPRQVINILNYEGGEIDIILAEVDLPVSKCFKMLKYIARNKDLRHIPIIMMSNRDEVSVVVKCLRLGAAEYLVKPLRTNELLNLWTHVWRRRRMLGLPEKNFFNDNFELVLSEPSDANTNSTTLLSDETDDRPKGNTNQETGTSKQLEYESNPSVAEPDQREKMEGVPGSALDASKKSSPRRAFSRPIKTNLRVAESSAFLAYVKPSTPATSSFDSELQRGGSRLDSLDNQGNCSSATDRSDTGTDVNIRSKEAFEMPAQYPMVWFSSSNMHMERSSEGHNDTSGTPPAYHFPFYYPGMVEHNMALSSAQDFQANINNAQAHTPPAMLPQYNVYPQCHGLPVIPSFQFNPSGMSTHSSHLPTQNVWSSASSTPPVPEETCSRSERRAAALAKFRLKRKERCFDKKVRYVNRKKLAETRPRVRGQFVRQPTNTDVISIGDDISEDEDDDPSSREVDMVSSPE

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGGGTGGCGGCGAGGGGGATCGCGTGGGTGTCGGGGGAGCCCAGCAGTTCGTGGACCGGAGCAAGGTGAGGATTCTTCTCTGCGACGGCGACGCCACCAGCTCCCGGGAGGTGCTGCGGCTCCTCTGCAACTGCTCATACCATGTGACCTGCGCCAAGTCTCCGCGGCAGGTGATCAATATCCTCAACTACGAGGGCGGCGAGATCGACATCATCCTGGCTGAGGTCGATCTTCCGGTCTCCAAGTGCTTCAAGATGCTCAAGTACATTGCCCGGAACAAGGACCTGCGTCACATCCCCATCATAATGATGTCCAACAGAGACGAGGTGTCTGTTGTTGTCAAGTGCTTGCGGCTTGGCGCCGCCGAGTACCTGGTGAAGCCGCTGCGCACGAATGAGTTGCTGAACCTTTGGACCCATGTGTGGCGGCGGAGACGGATGCTTGGTTTGCCCGAGAAAAACTTCTTCAATGACAACTTCGAGTTGGTGCTCTCAGAACCTAGCGATGCCAATACCAATAGCACCACCCTCCTCTCAGACGAGACCGACGATAGGCCTAAAGGAAACACGAATCAAGAAACAGGCACCTCAAAGCAACTTGAATACGAGTCTAATCCTTCGGTTGCTGAGCCCGACCAGAGAGAAAAGATGGAGGGTGTTCCAGGCTCTGCCCTAGATGCCAGTAAAAAATCCTCTCCAAGAAGAGCGTTTTCACGCCCTATAAAAACTAACCTGAGGGTTGCCGAGTCGTCTGCATTTCTAGCATATGTCAAGCCAAGCACCCCAGCCACCAGCTCATTTGACAGCGAATTACAAAGGGGTGGCAGCCGATTAGATTCCTTGGATAACCAGGGTAATTGCTCTAGCGCAACCGATAGAAGTGACACTGGTACTGATGTAAATATTCGGAGTAAAGAAGCTTTTGAGATGCCTGCGCAGTACCCTATGGTATGGTTTTCTTCCTCTAACATGCATATGGAGCGAAGCAGCGAAGGCCATAATGATACTTCAGGAACTCCACCTGCATACCATTTCCCATTTTATTACCCAGGGATGGTAGAGCACAACATGGCACTTTCGTCGGCGCAAGATTTTCAAGCAAACATAAACAATGCTCAAGCACATACACCGCCAGCGATGCTTCCTCAGTACAATGTTTATCCTCAGTGCCATGGTTTACCTGTGATACCATCATTTCAGTTTAATCCTTCTGGCATGAGTACGCACTCAAGTCATTTACCAACGCAAAATGTGTGGTCATCGGCATCGAGCACACCACCAGTGCCTGAGGAAACATGCAGTCGCTCTGAAAGGAGGGCTGCAGCACTTGCCAAGTTCAGGCTGAAGAGGAAAGAGCGCTGCTTTGACAAGAAGGTGAGGTATGTGAACCGGAAGAAACTCGCTGAAACGAGGCCAAGGGTGCGAGGTCAGTTTGTTAGACAGCCAACCAATACGGATGTCATCAGCATTGGAGATGATATATCTGAAGACGAAGATGACGATCCATCGTCCAGGGAGGTAGATATGGTTTCTTCTCCAGAG