| TFome pUT1454 | |
|---|---|
| Template | EC886186 |
| Genbank Insert ID | HQ858748 |
| Protein Name | ZmFHA16 |
| Species | Zea mays |
| TF Family | FHA |
| Gene id | GRMZM2G042627 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1454
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C49UP |
| Temperature (ºC) | 62.4 |
| Sequence
|
caccATGGCCGTCCCTCCT |
3' Primer
| Name | C49DN |
| Temperature (ºC) | |
| Sequence
|
ATC AAC TGG CGA GAT ACA GGG ATC |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MAVPPLLLVAVAVAALSILVLVVFAFRRCRRRRQRHPLQAAAVAPTPVAVQDEDIDRPLLSENQSDHSRQNISFLGSSVGEPSKIQTNRSNTSPRSHAIADTGRIYPAECCVTQGETHVINVENDTSEEFQLGSTLRRTPPPKWPTPDQKHRRRVSGDDNHNGSVPLKDNTYHSRLDLEVIAGPSHGISCSRQSSRPSMLPITLGRVPPSDLVLKDSEVSGKHAQINWNGKTLKWELVDMGSLNGTFLNSQAVHHPSAGSRHWGEPAELAHGDIITLGTSSKLSVQILLQNQRVPAGVGMASDPMVARRSGKKLPMEDISFCQCPLQGVEHFGLFGIFDGHGGDGAAKAVSKILPEHLGYILSHPETKERVQSYSDASDVLRYAFTLTEDTIDHQYEGCTGTALLIWFDQNKDCFAQCANLGDSACVMSVNGKTIDMTEDHRVTSATERARIARTGQPLRDGEARLSGLNLARMFGDKFLKEQDPRFSSEPYVSQAVRITKACTAFAVIASDGLWDVISTKRAVQLVVEGKERRSSGDATSAAARVASRVLDEARSLRTKDNTSVIFVDFDVLRRDPCISPVD
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCCGTCCCTCCTCTGCTGCTCGTCGCCGTTGCCGTAGCCGCGCTCTCCATCCTCGTCCTCGTCGTCTTCGCCTTCCGGCGATGCAGGCGGCGGCGGCAACGCCACCCGCTGCAGGCCGCGGCGGTGGCCCCGACCCCGGTCGCTGTCCAGGATGAGGACATAGATAGGCCTCTTCTTTCTGAAAATCAGAGCGATCACTCTAGACAGAATATTAGTTTTCTTGGAAGTTCTGTAGGAGAACCTTCGAAGATTCAAACAAATAGGAGTAACACTTCGCCTAGAAGTCATGCAATTGCTGATACAGGGAGGATTTATCCTGCTGAATGTTGTGTTACACAAGGAGAAACTCATGTAATCAATGTTGAAAATGATACTTCTGAAGAGTTCCAATTAGGAAGCACACTAAGACGTACACCGCCACCAAAATGGCCAACACCTGACCAGAAGCACAGAAGAAGGGTTTCTGGAGATGACAATCATAATGGAAGTGTTCCTTTGAAGGATAATACATATCATAGCCGCCTGGATTTAGAGGTTATAGCTGGTCCATCTCATGGAATAAGCTGTTCTCGGCAGTCAAGTAGACCTTCTATGCTCCCAATAACTCTTGGAAGGGTTCCCCCAAGTGATTTAGTGTTGAAGGACTCTGAAGTATCAGGGAAACATGCTCAGATAAACTGGAATGGAAAGACATTAAAATGGGAACTTGTGGACATGGGCAGCTTGAATGGAACTTTCTTGAACTCCCAGGCGGTTCACCATCCAAGTGCTGGGTCTAGGCATTGGGGTGAACCTGCTGAACTTGCACATGGTGATATTATAACTCTAGGGACTTCATCTAAACTGTCTGTTCAAATTTTACTGCAAAATCAACGAGTTCCTGCTGGTGTTGGTATGGCATCCGATCCAATGGTGGCACGTAGAAGTGGAAAGAAACTTCCAATGGAAGACATAAGCTTTTGCCAGTGTCCTCTGCAGGGTGTTGAACATTTTGGGCTCTTTGGAATTTTTGATGGACACGGTGGGGATGGAGCTGCTAAAGCTGTCAGCAAGATTCTTCCAGAACATCTGGGATACATTTTATCTCATCCTGAAACAAAAGAACGAGTGCAATCATATTCTGATGCTTCTGATGTTCTTAGATATGCTTTTACTTTGACAGAAGACACAATTGATCATCAGTACGAGGGATGTACAGGCACAGCACTTCTTATTTGGTTTGACCAGAATAAAGATTGTTTTGCCCAGTGCGCTAATCTTGGTGACTCTGCATGCGTCATGAGTGTCAACGGCAAGACGATCGATATGACTGAAGATCACCGGGTAACTAGCGCGACCGAAAGAGCTAGGATAGCAAGAACTGGGCAGCCCCTGAGAGACGGTGAAGCTCGTCTTAGCGGACTAAATCTTGCTAGGATGTTTGGTGACAAGTTTCTCAAGGAGCAGGACCCGCGCTTCAGCTCAGAACCATACGTGAGCCAAGCCGTTCGCATCACCAAAGCGTGCACAGCCTTCGCCGTCATTGCTAGCGACGGGCTCTGGGACGTCATCAGCACGAAAAGGGCGGTACAGCTTGTCGTCGAGGGAAAGGAGAGGAGGAGCTCCGGCGACGCCACCTCAGCGGCGGCCAGAGTAGCCAGCCGTGTGCTGGACGAAGCTCGGAGTCTGCGAACCAAGGACAACACATCCGTAATATTCGTAGATTTTGACGTCCTGAGAAGGGATCCCTGTATCTCGCCAGTTGAT
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