| TFome pUT1610 | |
|---|---|
| Template | |
| Genbank Insert ID | HQ858847 |
| Protein Name | |
| Species | Oryza sativa |
| TF Family | |
| Gene id | LOC_Os03g58830 |
| transcript | |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1610
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | |
| Temperature (ºC) | 67.8 |
| Sequence
|
caccatggaccccgcccccac |
3' Primer
| Name | R222DN |
| Temperature (ºC) | 63.2 |
| Sequence
|
tgtcactctttcataggtgccaccaatctgc |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MDPAPTLAAELWRTPYLGGGGGGGGGGRGLEAAASGVTEQSNGSRGGGGGGGAGRRRQREAPALEDDSSRIVSASGGGGGGQDLTDSEAKRFKASKSSGDNSSLRTEAETDSRNASKSGDQNPPPPEPPKQDYIHVRARRGQATDSHSLAERARREKISERMKILQDLVPGCNKVIGKASVLDEIINYIQALQRQVEFLSMKLEAVNAHVNNGIEAFPPKDFGAQVYNTAPGLTFDPQTPREYAQGSTPSEWLHMQIGGTYERVT
TFome clones do not contain stop codon at 3'end.
CDS
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AuGGACCCCGCCCCCACGCuCGCCGCCGAGCuCuGGCGAACGCCGuACCuCGGuGGCGGuGGuGGuGGAGGAGGAGGAGGGCGuGGGuuGGAGGCGGCGGCGuCCGGGGuCACGGAGCAGAGCAAuGGGAGCCGCGGGGGuGGCGGAGGAGGAGGCGCAGGGCGGAGGAGGCAGCGGGAGGCCCCCGCGCuCGAGGACGACuCGuCCAGGAuCGuCuCCGCuuCCGGAGGAGGAGGCGGCGGuCAGGAuuuGACuGAuuCAGAAGCAAAGCGuuuCAAGGCAAGuAAAuCAAGuGGAGAuAAuAGCAGuuuAAGAACAGAGGCuGAAACuGAuuCAAGAAAuGCuAGCAAGuCuGGuGAuCAAAAuCCGCCACCACCAGAACCACCAAAGCAAGACuACAuCCAuGuGAGGGCAAGAAGGGGuCAAGCuACuGACAGCCAuAGCCuuGCCGAAAGGGCACGuCGuGAGAAAAuAAGuGAGCGGAuGAAAAuuCuCCAGGAuCuuGuCCCuGGAuGCAACAAGGuuAuuGGGAAAGCAuCAGuACuuGAuGAGAuAAuuAAuuAuAuCCAGGCuuuGCAACGGCAAGuuGAGuuuuuGuCCAuGAAGCuuGAGGCuGuuAAuGCuCAuGuGAAuAAuGGAAuCGAAGCAuuuCCACCCAAAGAuuuuGGuGCuCAGGuAuACAACACGGCuCCuGGAuuGACGuuuGAuCCACAAACACCAAGAGAAuACGCCCAGGGAuCAACACCAuCGGAAuGGCuCCACAuGCAGAuuGGuGGCACCuAuGAAAGAGuGACA
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