TFome clone pUT1611

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)	58.2
Sequence Copy to clipboard	caccatggcgtctcctgatg

3' Primer

Name	R246DN
Temperature (ºC)	57.6
Sequence Copy to clipboard	aggatcgaagccaaacatgtcg

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

Copy to clipboard

MASPDGGVGDGGAEPHEVMDDLLEMREQAAMLHSMLHGTSPSSCAAAASTRQLNQLIDGVMSRLQSSSLSVMSPGGGGGRRGSGGRKKKGAKAVAGPHRRSSSGRRRSKSPFVRMVTTKELEDGRQWRKYGQKHIQDSPNNPRSYYRCTHRPDQGCMATKQVQTSESNSSEFVISYYGEHTCSDPSTIPFVVEAEAPAADYANLISFGSSGGASTSRVDPLRQSRHRLMAEAVDPTPSCSFANCHSPVLSSECASEAAALSSSLPLSAVVGSAVTTPSTSIVGSAPADYDWPSGLAGGDMAGSFPSSPSSLGFMTGSFGNLPGDDDDMFGFDP

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

AuGGCGuCuCCuGAuGGuGGCGuuGGAGACGGCGGCGCGGAGCCACAuGAAGuuAuGGACGACCuGCuCGAGAuGCGCGAGCAAGCGGCGAuGCuCCACAGCAuGCuACAuGGGACAuCGCCGuCGAGCuGuGCGGCGGCGGCGAGCACCAGGCAGCuGAAuCAGCuGAuAGACGGGGuGAuGAGCAGGCuGCAGuCCAGCuCGCuGuCGGuGAuGuCGCCuGGAGGAGGAGGAGGAAGAAGAGGAuCGGGCGGGAGGAAGAAGAAAGGCGCCAAGGCCGuGGCCGGGCCGCACAGGCGGAGCAGCuCCGGuAGAAGAAGAuCGAAAAGuCCuuuuGuCAGGAuGGuCACGACCAAGGAGCuGGAGGACGGCAGGCAAuGGCGAAAGuACGGGCAGAAACAuAuACAGGACuCCCCAAAuAAuCCAAGGAGCuACuACAGGuGCACGCAuAGGCCAGACCAAGGuuGuAuGGCCACAAAGCAAGuACAGACCuCCGAGuCuAACuCGuCGGAGuuCGuCAuCAGCuACuACGGuGAGCACACCuGCAGCGACCCuuCCACCAuCCCGuuCGuCGuCGAGGCCGAAGCuCCCGCuGCAGACuACGCGAACCuCAuCAGCuuCGGAuCCAGCGGCGGCGCGAGCACAAGCAGAGuCGACCCuCuuCGuCAGAGCCGCCAuCGuuuGAuGGCAGAAGCCGuuGAuCCGACGCCGuCCuGCAGCuuCGCCAACuGCCACuCuCCGGuACuGuCCuCGGAGuGCGCCAGCGAGGCGGCGGCGCuCAGCAGCAGCCuGCCGCuCuCCGCCGuGGuGGGAuCGGCGGuGACAACGCCGuCGACGuCGAuCGuGGGuuCAGCGCCuGCGGAuuACGACuGGCCGuCAGGGCuCGCCGGAGGuGACAuGGCCGGCAGCuuCCCGuCCuCuCCCAGCAGCCuCGGGuuCAuGACuGGCuCCuuuGGGAAuCuuCCuGGGGAuGACGACGACAuGuuuGGCuuCGAuCCu