TFome pUT1611

Placeholder text:
"TFome" is used as a synonym for "clone".
"TFome" is short for "transcription factor open reading frame".

5' Primer

Name
Temperature (ºC)	58.2
Sequence Copy to clipboard	caccatggcgtctcctgatg

3' Primer

Name	R246DN
Temperature (ºC)	57.6
Sequence Copy to clipboard	aggatcgaagccaaacatgtcg

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MASPDGGVGDGGAEPHEVMDDLLEMREQAAMLHSMLHGTSPSSCAAAASTRQLNQLIDGVMSRLQSSSLSVMSPGGGGGRRGSGGRKKKGAKAVAGPHRRSSSGRRRSKSPFVRMVTTKELEDGRQWRKYGQKHIQDSPNNPRSYYRCTHRPDQGCMATKQVQTSESNSSEFVISYYGEHTCSDPSTIPFVVEAEAPAADYANLISFGSSGGASTSRVDPLRQSRHRLMAEAVDPTPSCSFANCHSPVLSSECASEAAALSSSLPLSAVVGSAVTTPSTSIVGSAPADYDWPSGLAGGDMAGSFPSSPSSLGFMTGSFGNLPGDDDDMFGFDP

TFome clones do not contain stop codon at 3'end.

CDS

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AuGGCGuCuCCuGAuGGuGGCGuuGGAGACGGCGGCGCGGAGCCACAuGAAGuuAuGGACGACCuGCuCGAGAuGCGCGAGCAAGCGGCGAuGCuCCACAGCAuGCuACAuGGGACAuCGCCGuCGAGCuGuGCGGCGGCGGCGAGCACCAGGCAGCuGAAuCAGCuGAuAGACGGGGuGAuGAGCAGGCuGCAGuCCAGCuCGCuGuCGGuGAuGuCGCCuGGAGGAGGAGGAGGAAGAAGAGGAuCGGGCGGGAGGAAGAAGAAAGGCGCCAAGGCCGuGGCCGGGCCGCACAGGCGGAGCAGCuCCGGuAGAAGAAGAuCGAAAAGuCCuuuuGuCAGGAuGGuCACGACCAAGGAGCuGGAGGACGGCAGGCAAuGGCGAAAGuACGGGCAGAAACAuAuACAGGACuCCCCAAAuAAuCCAAGGAGCuACuACAGGuGCACGCAuAGGCCAGACCAAGGuuGuAuGGCCACAAAGCAAGuACAGACCuCCGAGuCuAACuCGuCGGAGuuCGuCAuCAGCuACuACGGuGAGCACACCuGCAGCGACCCuuCCACCAuCCCGuuCGuCGuCGAGGCCGAAGCuCCCGCuGCAGACuACGCGAACCuCAuCAGCuuCGGAuCCAGCGGCGGCGCGAGCACAAGCAGAGuCGACCCuCuuCGuCAGAGCCGCCAuCGuuuGAuGGCAGAAGCCGuuGAuCCGACGCCGuCCuGCAGCuuCGCCAACuGCCACuCuCCGGuACuGuCCuCGGAGuGCGCCAGCGAGGCGGCGGCGCuCAGCAGCAGCCuGCCGCuCuCCGCCGuGGuGGGAuCGGCGGuGACAACGCCGuCGACGuCGAuCGuGGGuuCAGCGCCuGCGGAuuACGACuGGCCGuCAGGGCuCGCCGGAGGuGACAuGGCCGGCAGCuuCCCGuCCuCuCCCAGCAGCCuCGGGuuCAuGACuGGCuCCuuuGGGAAuCuuCCuGGGGAuGACGACGACAuGuuuGGCuuCGAuCCu