TFome clone pUT1612

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)	60.9
Sequence Copy to clipboard	caccatggagcttgacgaggagt

3' Primer

Name	R230DN
Temperature (ºC)	60.3
Sequence Copy to clipboard	cagacaccttccgccatagcc

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MELDEESFLDELMSLRRDGSAPWQAPPYPGGGGGGGGGGMMMSDLLFYGGDGGSAEARGGMDASPFQELASMAAPPPQHPHEEFNFDCLSEVCNPYRSCGAQLVPSEAASQTQTQLTPLRDAMVAEEETSGDKALLHGGGGSSSPTFMFGGGAGESSEMMAGIRGVGGGVHPRSKLHGTPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYVKELTERIKTLEEEIGVTPEELDLLNTMKDSSSGNNNEMLVRNSTKFDVENRGSGNTRIEICCPANPGVLLSTVSALEVLGLEIEQCVVSCFSDFDMQASCLQEDGKRQVVSTDENKQTLFRSAGYGGRCL

TFome clones do not contain stop codon at 3'end.

CDS

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AuGGAGCuuGACGAGGAGuCCuuCuuGGAuGAGCuCAuGuCGCuGCGGCGuGAuGGAuCGGCGCCGuGGCAGGCGCCGCCGuACCCGGGuGGCGGCGGCGGCGGCGGCGGuGGCGGCAuGAuGAuGAGuGACCuCCuCuuCuACGGCGGCGACGGCGGCAGCGCGGAGGCGAGGGGCGGGAuGGACGCGuCGCCGuuCCAGGAGCuGGCGuCCAuGGCGGCGCCGCCGCCuCAGCAuCCGCACGAGGAGuuCAACuuCGACuGCuuGAGCGAGGuCuGCAACCCGuACCGCAGCuGCGGCGCCCAGCuCGuCCCCuCGGAGGCGGCuAGCCAGACGCAGACGCAGCuCACuCCuCuCCGCGACGCAAuGGuGGCGGAGGAGGAGACGAGCGGCGACAAGGCGCuGCuCCAuGGCGGCGGCGGCuCGuCGuCGCCGACGuuCAuGuuuGGAGGuGGCGCCGGAGAGAGCuCGGAGAuGAuGGCCGGCAuCAGAGGCGuCGGCGGCGGCGuCCACCCGCGGAGCAAGCuCCACGGCACGCCGuCGAAGAACCuCAuGGCCGAGAGGCGGCGuCGCAAGCGGCuCAACGACCGGCuGuCCAuGCuCCGGuCCAuCGuGCCCAAGAuuAGCAAGAuGGACAGGACCuCGAuCCuuGGuGACACCAuCGACuACGuGAAGGAGCuGACGGAGAGGAuCAAAACCCuCGAGGAGGAGAuCGGCGuCACGCCGGAGGAGCuCGACCuGuuGAACACGAuGAAGGAuuCCuCCAGCGGCAACAACAACGAGAuGCuCGuGAGGAAuuCCACCAAGuuCGAuGuCGAGAACCGGGGCAGCGGGAACACGAGGAuCGAGAuAuGCuGCCCGGCGAACCCCGGCGuGCuGCuGuCGACGGuGAGCGCGCuGGAGGuGCuGGGGCuAGAGAuCGAGCAGuGCGuCGuGAGCuGCuuCAGuGACuuuGACAuGCAGGCCuCCuGCuuACAAGAGGACGGGAAGAGGCAAGuuGuCAGCACCGACGAGAACAAGCAGACAuuGuuCAGGAGCGCAGGCuAuGGCGGAAGGuGuCuG