TFome clone pUT1613

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)	65.7
Sequence Copy to clipboard	caccatggatccgcgcagcagc

3' Primer

Name	R213DN
Temperature (ºC)	61.1
Sequence Copy to clipboard	TGT CTG TAC TGT CGA TCG TTG ATC ATC AGC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MDPRSSQAQEDGFFHPRDGACPADSSGKTECKTQGSIATRKVQKADREKMRRDRLNEQFQELGSTLDPDRPRNDKATILSDAIQMLKDLTSQVNKLKAEYTSLSEEARELTQEKNELRDEKVSLKFEVDNLNTQYQQRMRVLYPWTGMEPSVVIGPPLPYPFSVPVPVPVPIPSGAVPMHPQLQAYPYFRNQTSGTVSNPCTPYMAYTQPIHPPTDQLSNQFSAPVQHSSSNRSHSMAQDSRSKSSALQQVSCRGKHDDFDDVATDLELKTPGSSAPLQSEIANKDSSSDLKKKQQFIQETKGSSLTEGSSSSSRCSSSGPPDVSNSIEGGSVADDQRSTVQT

TFome clones do not contain stop codon at 3'end.

CDS

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AuGGAuCCGCGCAGCAGCCAGGCGCAAGAGGACGGCuuCuuCCACCCACGCGACGGCGCGuGCCCCGCCGACuCCAGCGGAAAGACAGAAuGuAAAACCCAGGGuuCAAuuGCCACGCGCAAGGuuCAAAAGGCuGAuCGuGAGAAAAuGCGAAGAGAuAGAuuGAAuGAGCAGuuCCAGGAACuuGGAAGCACACuuGAuCCAGAuCGACCCAGGAAuGAuAAGGCAACuAuCCuuAGuGAuGCAAuuCAAAuGCuGAAAGAuuuGACGuCCCAGGuGAACAAGCuuAAAGCuGAAuAuACuuCACuCuCAGAAGAAGCACGuGAGuuGACuCAGGAAAAGAAuGAGCuCAGAGAuGAGAAAGuuuCACuGAAGuuuGAGGuuGAuAAuCuGAAuACCCAGuAuCAGCAACGAAuGCGuGuGCuGuACCCuuGGACuGGAAuGGAGCCuuCuGuuGuCAuuGGGCCACCCCuGCCAuAuCCuuuuuCAGuCCCAGuCCCAGuCCCAGuCCCuAuACCuuCAGGuGCuGuuCCuAuGCACCCACAGCuCCAGGCAuACCCuuACuuCCGAAAuCAAACuuCuGGAACCGuCuCCAAuCCAuGCACACCuuACAuGGCAuACACuCAACCCAuCCAuCCuCCuACuGAuCAACuAuCuAACCAAuuCAGuGCuCCAGuuCAGCAuuCAAGuAGuAAuCGAuCuCACuCuAuGGCCCAAGACuCuAGAAGCAAGuCCuCuGCACuGCAGCAAGuAAGuuGuAGAGGGAAACAuGAuGACuuuGAuGACGuuGCAACuGAuCuGGAACuAAAAACuCCuGGuuCCuCAGCCCCuCuACAAuCAGAGAuuGCCAACAAGGAuuCCuCuuCuGACuuGAAAAAAAAGCAGCAGuuCAuACAGGAAACuAAAGGuAGuuCuCuuACAGAAGGCAGuAGuAGuuCAAGCAGAuGCuCGuCCAGuGGuCCuCCAGAuGuCuCuAACAGuAuuGAAGGuGGAuCAGuuGCuGAuGAuCAACGAuCGACAGuACAGACA