| PCR Condition:
|
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s |
| Nucleotide Sequence:
|
AuGGAGAACuuCCCGAuACuCuuuGCGACGCAGCCGACGuCGuCuuCuACCuCCAGCuCCuACCACuuCAuGAGCuCCuCuuCCGGCAGCCAuGACCACCGCCACCACCAuGGACuACAGGCAGGCGGCAACGGCGGuGGCGGuGGuGGAAGCuuGAGCCAuGGCuuGuuCAuGGGGuCGuCGuCGuCGuCAAuuAGGAuGGAGGAGCuCuCCAACuCCAAGCAGGCCGGCGACGuCGuCGuCGACGGuGGuGCAACGAGGAGCCCACACGGCGGCGACGGAGACGGAGCuGCGGGCGACGACGGCGGCGAuGCGCAGGCAGCCGCCGCCGGAGGGAGGAAGAAGGGGGAGAAGAAGGAGCGGCGGCCGCGGuuCGCGuuCCAGACGCGCAGCCAGGuCGACAuCCuCGACGACGGCuACCGGuGGAGGAAGuACGGCCAGAAGGCCGuCAAGAACAACAAAuuCCCAAGGAGCuACuACCGGuGCACGCACCAAGGGuGCAACGuGAAGAAGCAGGuGCAGCGGCuGuCGCGGGACGAGACGGuGGuGGuGACGACGuACGAGGGCACGCACACGCACCCCAuCGAGAAAuCCAACGACAACuuCGAGCACAuCCuCACCCAAAuGCACAuCuACuCCGGCCuCACGCCuuCAuCGGCuGCGCAuGCCuCAuCCuCCuCACCuCuCuuCCCuuCAGCuGCuGCuGCuGCuuCCCACAuGuuCCAG |
|
|
TFome clones do not contain stop codon at 3'end.
|
| translation:
|
MENFPILFATQPTSSSTSSSYHFMSSSSGSHDHRHHHGLQAGGNGGGGGGSLSHGLFMGSSSSSIRMEELSNSKQAGDVVVDGGATRSPHGGDGDGAAGDDGGDAQAAAAGGRKKGEKKERRPRFAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCNVKKQVQRLSRDETVVVTTYEGTHTHPIEKSNDNFEHILTQMHIYSGLTPSSAAHASSSSPLFPSAAAAASHMFQ
|
