TFome clone pUT1616

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)	63.8
Sequence Copy to clipboard	caccATGGAGAGCTTGTGTGGGCAG

3' Primer

Name	R206DN
Temperature (ºC)	62.4
Sequence Copy to clipboard	ATGAAAGAAGCAGTGGCTGCTCATCTCG

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MESLCGQLEHEHEQQLHQSSSSSLLPYCFLSAPEPLPVPGFPAGCSNLEEKAAAAMAAYEYESSSCSSLDPTSMPMVYSPIVLQPQECPLSFVFDNAAAAAGDNKWVPGIQGSCPCSLGSTQDMDASWGKSRKHKRSNVGLKGLEEKKARRVVLHQHDDDVKKKAKEAAGGEPPAGYIHVRARRGQATDSHSLAERVRREKISERMKMLQSLVPGCDKVTGKALMLDEIISYVQSLQNQVEFLSMKLASLSPLMYEFGPGIDMHPDVLRQLAKMPHEMVQCMGQMGSTGISLQGLGGGPTGFAQDGSSHINMVVMQVGEQGQQQGSLHQVEMSSHCFFH

TFome clones do not contain stop codon at 3'end.

CDS

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AuGGAGAGCuuGuGuGGGCAGCuuGAGCAuGAACAuGAGCAGCAGCuGCACCAAuCCuCCuCAuCuuCACuuCuCCCCuACuGCuuCCuuuCuGCuCCAGAGCCuCuuCCuGuCCCAGGCuuCCCuGCCGGCuGCAGCAACCuuGAAGAGAAGGCAGCAGCAGCCAuGGCGGCCuAuGAGuAuGAGAGCAGCAGCuGCAGCuCCCuuGACCCCACCuCAAuGCCAAuGGuGuACuCACCAAuAGuGCuGCAGCCACAGGAGuGCCCuCuuuCCuuuGuCuuuGACAAuGCCGCuGCCGCuGCCGGCGACAACAAAuGGGuGCCuGGCAuuCAAGGAAGuuGCCCuuGuuCAuuGGGCuCAACuCAAGAuAuGGAuGCAAGuuGGGGCAAGAGuAGAAAGCACAAGAGGAGCAAuGuGGGCuuGAAGGGACuuGAGGAGAAAAAGGCAAGAAGGGuGGuACuACAuCAACAuGAuGAuGAuGuGAAGAAGAAGGCCAAGGAGGCAGCAGGuGGGGAGCCACCAGCuGGGuACAuuCAuGuGAGGGCAAGGAGAGGCCAAGCAACAGACAGCCACAGCCuuGCuGAGAGGGuGAGAAGGGAGAAGAuCAGuGAGAGGAuGAAGAuGCuGCAAAGCCuGGuuCCuGGuuGuGACAAGGuCACAGGGAAAGCCCuCAuGCuAGAuGAGAuCAuCAGCuAuGuGCAAuCuCuGCAGAACCAAGuuGAGuuuCuGuCCAuGAAGCuuGCCuCCCuuAGCCCuuuGAuGuAuGAGuuuGGACCGGGCAuuGAuAuGCACCCAGAuGuuCuuCGACAGCuuGCAAAGAuGCCACAuGAGAuGGuGCAGuGCAuGGGCCAAAuGGGGAGuACAGGCAuuuCACuCCAAGGACuAGGAGGAGGACCCACAGGCuuuGCuCAGGAuGGCAGCAGCCACAuuAACAuGGuGGuGAuGCAAGuuGGAGAGCAAGGCCAGCAGCAGGGCuCuCuuCACCAGGuCGAGAuGAGCAGCCACuGCuuCuuuCAu