TFome clone pUT1620

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)	60.6
Sequence Copy to clipboard	caccatgtcttctggaggaggagg

3' Primer

Name	R243DN
Temperature (ºC)	61.4
Sequence Copy to clipboard	tgggttgctgctgggcattg

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MSSGGGGGGGGDRHGPYHQHGHLGRGEGADYVYSSSDMESFFFSQPGGVGIGGGGGGVVGAGGADEIMPYSSITDYLQGLLDPSGLARHLDVACPSSQDTVVKQELSVDVTSHDSQGTGGVAGEGVAQATPNSSASFSSSDGEAEGGKSSRRCKKGQAKAEEEDDKDEEDGENSKKPNKPKKKAEKRQRQPRVAFLTKSEVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTPKCGVKKRVERSYQDPSTVITTYEGQHTHHSPASLRGGGGGVGIVGGHHHHHLFMPGVHGLPPSHLMPAGFHPELMGLMHHHPAMAAAAANPSMYFPGVAASAPPPPAVAGGGAMPPNDHPPLQQHHFTDYALLQDLFPSTMPSSNP

TFome clones do not contain stop codon at 3'end.

CDS

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AuGuCuuCuGGAGGAGGAGGAGGAGGAGGAGGAGAuCGCCAuGGCCCCuACCACCAGCACGGGCACCuCGGCCGCGGCGAAGGCGCuGAuuACGuGuACAGCAGCAGCGACAuGGAGAGCuuCuuCuuCAGCCAACCCGGGGGCGuCGGCAuCGGCGGGGGuGGuGGuGGCGuCGuCGGCGCCGGCGGuGCCGACGAGAuCAuGCCGuACuCCAGCAuCACGGACuACCuGCAGGGGuuAuuGGACCCCuCCGGGCuAGCuCGGCACCuCGACGuGGCGuGCCCGuCGuCuCAGGACACGGuGGuCAAGCAGGAGCuGuCGGuGGAuGuGACGAGCCACGACAGCCAGGGCACCGGCGGCGuCGCCGGAGAAGGCGuCGCGCAGGCCACGCCGAACuCGuCGGCGuCCuuCuCGuCCAGCGACGGAGAGGCGGAGGGCGGCAAAuCGuCACGCCGGuGCAAGAAGGGuCAGGCGAAGGCGGAGGAGGAGGACGACAAGGAuGAGGAGGAuGGAGAGAACuCCAAGAAACCGAACAAGCCCAAGAAGAAAGCuGAGAAGAGGCAGCGGCAGCCuCGCGuGGCGuuCCuCACCAAGAGCGAGGuuGAuCACCuCGAGGACGGCuACCGGuGGCGCAAGuACGGCCAGAAGGCAGuCAAGAACAGCCCAuACCCGAGGAGCuACuACCGGuGCACGACGCCCAAGuGCGGuGuGAAGAAGCGGGuGGAGCGGuCGuACCAGGACCCCuCCACGGuGAuCACCACGuACGAAGGGCAGCACACGCACCACAGCCCGGCGAGCCuCCGCGGCGGCGGCGGCGGCGuCGGCAuCGuCGGAGGGCACCACCACCACCACCuCuuCAuGCCCGGCGuGCAuGGCCuGCCGCCGuCGCACCuCAuGCCGGCGGGGuuCCACCCuGAGCuGAuGGGCCuCAuGCACCACCACCCGGCCAuGGCCGCCGCCGCCGCAAACCCuAGCAuGuACuuCCCGGGCGuAGCuGCuuCuGCuCCGCCGCCuCCuGCuGuGGCuGGCGGCGGuGCAAuGCCGCCCAACGAuCAuCCuCCuCuuCAGCAGCAuCACuuCACuGACuACGCCCuGCuGCAAGACCuuuuCCCuuCCACAAuGCCCAGCAGCAACCCA