| TFome pUT1665 | |
|---|---|
| Template | |
| Genbank Insert ID | HQ858858 |
| Protein Name | |
| Species | Oryza sativa |
| TF Family | |
| Gene id | LOC_Os01g02110 |
| transcript | |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1665
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | |
| Temperature (ºC) | 61.1 |
| Sequence
|
caccatgatggcagctcaggc |
3' Primer
| Name | R240DN |
| Temperature (ºC) | 60.4 |
| Sequence
|
tttgtttggatgctgagaagataggatggc |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MMAAQASSKRGMLLPREAVLYDDEPSMPLEILGYHGNGVGGGGCVDADYYYSWSGSSSSSSSSVLSFDQAAVGGSGGGCARQLAFHPGGDDDDCAMWMDAAAGAMVENTSVVAGGGNNYCHRLQFHGGAAGFGLASPGSSVVDNGLEIHESNVSKPPPPAAKKRACPSGEARAAGKKQCRKGSKPNKAASASSPSPSPSPSPSPNKEQPQSAAAKVRRERISERLKVLQDLVPNGTKVDLVTMLEKAINYVKFLQLQVKVLATDEFWPAQGGKAPELSQVKDALDAILSSQHPNK
TFome clones do not contain stop codon at 3'end.
CDS
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AuGAuGGCAGCuCAGGCAAGCAGCAAGCGCGGCAuGCuGCuGCCACGGGAGGCGGuGCuCuACGACGACGAGCCCuCCAuGCCGCuGGAGAuCuuGGGCuACCACGGCAAuGGCGuCGGCGGCGGuGGCuGCGuuGACGCCGAuuACuACuACAGCuGGuCGGGGuCCAGCuCCAGCuCCAGCuCGuCGGuGCuCAGCuuuGACCAGGCGGCGGuCGGCGGCAGCGGCGGCGGCuGCGCCCGGCAGCuGGCuuuCCAuCCCGGCGGCGACGACGACGACuGCGCCAuGuGGAuGGACGCCGCCGCCGGCGCCAuGGuCGAGAACACGuCuGuCGuCGCCGGCGGCGGCAACAACuACuGuCAuCGCCuGCAGuuCCACGGCGGCGCCGCCGGuuuCGGACuCGCGAGCCCAGGCuCGuCGGuCGuuGACAACGGCCuCGAAAuCCACGAGAGCAACGuCAGCAAGCCGCCACCGCCGGCAGCCAAGAAGCGCGCAuGCCCGAGCGGCGAGGCGAGAGCAGCGGGGAAGAAGCAGuGCAGGAAAGGGAGCAAGCCAAACAAGGCuGCuuCuGCuuCuuCuCCuuCuCCuuCuCCuuCuCCuuCuCCuuCuCCuAACAAGGAACAACCuCAAAGCGCCGCuGCAAAGGuAAGAAGAGAGCGGAuCAGuGAGAGGCuCAAAGuuCuuCAGGAuCuCGuGCCuAAuGGCACAAAGGuAGACuuGGuCACCAuGCuAGAAAAGGCGAuCAACuACGuCAAAuuCCuCCAGCuGCAAGuGAAGGuuuuGGCuACuGAuGAGuuCuGGCCGGCACAAGGAGGGAAAGCACCAGAGCuCuCuCAAGuCAAGGACGCCuuGGACGCCAuCCuAuCuuCuCAGCAuCCAAACAAA
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