TFome clone pUT1671

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)	63.0
Sequence Copy to clipboard	caccatggaggactcgagcctgttc

3' Primer

Name	R234DN
Temperature (ºC)	60.4
Sequence Copy to clipboard	gctttttgtttctcgcgcgctatttct

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MEDSSLFMEWAMETLQHLHPLPATPPPAGGGYAGDNATFPSLQALRESSVSQNGMAPPEPTAHEGHRASNSWSSGDTDSVSGGGGGAVMEHDGWSTSPNSVRCAAGGGGGGGGGGLWPVSWNFSSAMTQPCNDQATPSNPPTTTRARYGGGGVRYLPAAVSPSPSAQTRRASSKGNGGGGSGSSSAAPYAQEHIIAERKRREKINQRFIELSTVIPGLKKMDKATILSDAVRYVKEMQEKLSELEQHQNGGVESAILLKKPCIATSSSDGGCPAASSAVAGSSSSGTARSSLPEIEAKISHGNVMVRIHGENNGKGSLVRLLAAVEGLHLGITHTNVMPFSACTAIITIMAKVEDGVSVTAEDIVGKLNTVLQQNSRNSARETKS

TFome clones do not contain stop codon at 3'end.

CDS

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AuGGAGGACuCGAGCCuGuuCAuGGAGuGGGCCAuGGAGACGCuGCAGCACCuGCACCCGCuGCCGGCGACGCCGCCGCCuGCCGGCGGCGGGuACGCCGGCGACAACGCGACuuuuCCGuCGCuCCAAGCGCuCCGCGAGuCGuCGGuGuCGCAGAACGGCAuGGCCCCGCCGGAGCCAACGGCGCACGAAGGCCACCGAGCCuCCAACAGCuGGAGCuCGGGCGACACGGACAGCGuGuCCGGuGGCGGCGGCGGCGCGGuCAuGGAGCACGACGGAuGGuCGACGuCGCCAAAuuCGGuCAGGuGCGCCGCuGGCGGCGGGGGCGGCGGCGGCGGCGGCGGCCuCuGGCCCGuGAGCuGGAACuuCuCGuCGGCCAuGACGCAGCCGuGCAACGAuCAGGCCACGCCGuCGAACCCGCCGACGACGACGAGAGCGCGCuACGGCGGCGGCGGCGuuCGAuACCuGCCGGCCGCCGuGuCGCCAuCGCCAuCuGCACAGACACGGAGAGCCuCCuCCAAAGGCAACGGCGGCGGuGGCuCCGGCuCCuCGuCGGCGGCGCCGuACGCGCAGGAGCACAuCAuCGCGGAGCGCAAGCGCCGGGAGAAGAuCAACCAGCGGuuCAuCGAGCuCuCCACCGuCAuCCCGGGCCuCAAGAAGAuGGACAAGGCGACGAuCCuCuCCGACGCGGuGAGGuACGuGAAGGAGAuGCAGGAGAAGCuCAGCGAGCuCGAGCAACACCAGAACGGCGGCGuCGAGuCGGCAAuACuCCuCAAGAAGCCGuGCAuAGCCACGuCGuCGuCGGACGGCGGCuGCCCCGCAGCGuCAuCGGCCGuGGCuGGAAGCAGCAGCAGCGGCACGGCGAGAAGCuCGCuGCCGGAGAuCGAGGCGAAGAuCuCGCACGGCAACGuGAuGGuGAGGAuCCACGGCGAGAAuAAuGGCAAGGGGuCGCuGGuGCGGCuGCuCGCCGCGGuuGAAGGGCuCCACCuCGGCAuCACGCACACCAAuGuCAuGCCCuuCuCGGCAuGCACCGCCAuCAuAACCAuCAuGGCAAAGGuGGAGGACGGGGuGAGCGuCACAGCuGAGGACAuuGuAGGGAAGCuAAACACuGuGCuCCAGCAAAACAGCAGAAAuAGCGCGCGAGAAACAAAAAGC