PCR Condition:
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Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s |
Nucleotide Sequence:
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AuGGAGGACuCGAGCCuGuuCAuGGAGuGGGCCAuGGAGACGCuGCAGCACCuGCACCCGCuGCCGGCGACGCCGCCGCCuGCCGGCGGCGGGuACGCCGGCGACAACGCGACuuuuCCGuCGCuCCAAGCGCuCCGCGAGuCGuCGGuGuCGCAGAACGGCAuGGCCCCGCCGGAGCCAACGGCGCACGAAGGCCACCGAGCCuCCAACAGCuGGAGCuCGGGCGACACGGACAGCGuGuCCGGuGGCGGCGGCGGCGCGGuCAuGGAGCACGACGGAuGGuCGACGuCGCCAAAuuCGGuCAGGuGCGCCGCuGGCGGCGGGGGCGGCGGCGGCGGCGGCGGCCuCuGGCCCGuGAGCuGGAACuuCuCGuCGGCCAuGACGCAGCCGuGCAACGAuCAGGCCACGCCGuCGAACCCGCCGACGACGACGAGAGCGCGCuACGGCGGCGGCGGCGuuCGAuACCuGCCGGCCGCCGuGuCGCCAuCGCCAuCuGCACAGACACGGAGAGCCuCCuCCAAAGGCAACGGCGGCGGuGGCuCCGGCuCCuCGuCGGCGGCGCCGuACGCGCAGGAGCACAuCAuCGCGGAGCGCAAGCGCCGGGAGAAGAuCAACCAGCGGuuCAuCGAGCuCuCCACCGuCAuCCCGGGCCuCAAGAAGAuGGACAAGGCGACGAuCCuCuCCGACGCGGuGAGGuACGuGAAGGAGAuGCAGGAGAAGCuCAGCGAGCuCGAGCAACACCAGAACGGCGGCGuCGAGuCGGCAAuACuCCuCAAGAAGCCGuGCAuAGCCACGuCGuCGuCGGACGGCGGCuGCCCCGCAGCGuCAuCGGCCGuGGCuGGAAGCAGCAGCAGCGGCACGGCGAGAAGCuCGCuGCCGGAGAuCGAGGCGAAGAuCuCGCACGGCAACGuGAuGGuGAGGAuCCACGGCGAGAAuAAuGGCAAGGGGuCGCuGGuGCGGCuGCuCGCCGCGGuuGAAGGGCuCCACCuCGGCAuCACGCACACCAAuGuCAuGCCCuuCuCGGCAuGCACCGCCAuCAuAACCAuCAuGGCAAAGGuGGAGGACGGGGuGAGCGuCACAGCuGAGGACAuuGuAGGGAAGCuAAACACuGuGCuCCAGCAAAACAGCAGAAAuAGCGCGCGAGAAACAAAAAGC |
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TFome clones do not contain stop codon at 3'end.
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translation:
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MEDSSLFMEWAMETLQHLHPLPATPPPAGGGYAGDNATFPSLQALRESSVSQNGMAPPEPTAHEGHRASNSWSSGDTDSVSGGGGGAVMEHDGWSTSPNSVRCAAGGGGGGGGGGLWPVSWNFSSAMTQPCNDQATPSNPPTTTRARYGGGGVRYLPAAVSPSPSAQTRRASSKGNGGGGSGSSSAAPYAQEHIIAERKRREKINQRFIELSTVIPGLKKMDKATILSDAVRYVKEMQEKLSELEQHQNGGVESAILLKKPCIATSSSDGGCPAASSAVAGSSSSGTARSSLPEIEAKISHGNVMVRIHGENNGKGSLVRLLAAVEGLHLGITHTNVMPFSACTAIITIMAKVEDGVSVTAEDIVGKLNTVLQQNSRNSARETKS
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