TFome clone pUT1675

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name
Temperature (ºC)	56.2
Sequence Copy to clipboard	caccatggcgttagaggc

3' Primer

Name	R235DN
Temperature (ºC)	58.2
Sequence Copy to clipboard	gctgatggagaggacggc

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MALEAVVFPQGHFGYGCGRDSPAYGMPWCDVLAAAGGGGGFGEFYGVDEWDDQLQVASVDEWEVASKDNSDASTEGKAAAAERAEPVAAGRRKRRRTKVVKNKEEIECQRMTHIAVERNRRRQMNEYLAVLRSLMPASYSQRGDQASIVGGAINYVKELEQLLQSLEVQKSLKNRSGAMDAAGDSPFAGFFSFPQYSTSPRTGCSAAASAGSSGSASSVVMDDTAGSAESGRQSAAIADIEVTMVEGHASLKVLARRRPKQLLKLVVGLQQLRIPPLHLNVTTVDAMVLYSFSLKVEDDSKLGSVEDIATAVHQILGSIQQQEAVLSIS

TFome clones do not contain stop codon at 3'end.

CDS

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AuGGCGuuAGAGGCCGuGGuGuuuCCGCAGGGCCAuuuCGGCuACGGGuGCGGAAGGGACuCGCCGGCGuACGGGAuGCCGuGGuGCGACGuCCuGGCuGCuGCCGGCGGCGGCGGCGGCuuCGGuGAGuuuuACGGAGuGGACGAGuGGGAuGACCAGCuGCAGGuCGCCuCCGuGGAuGAGuGGGAGGuGGCAuCCAAGGAuAACuCGGAuGCuuCCACGGAGGGCAAGGCGGCGGCGGCGGAGCGGGCGGAGCCGGuGGCCGCCGGGAGGAGGAAACGGAGGCGGACGAAGGuCGuCAAGAACAAGGAGGAGAuCGAGuGCCAGCGGAuGACCCACAuuGCCGuCGAGCGCAACCGCCGCCGCCAGAuGAACGAGuACCuCGCCGuGCuCCGCuCCCuCAuGCCGGCGuCCuACuCGCAGAGGGGuGAuCAAGCAuCGAuCGuCGGAGGAGCAAuCAACuACGuGAAGGAGCuGGAGCAGCuACuGCAGuCGCuGGAAGuCCAAAAGAGCCuCAAGAACCGCAGCGGCGCCAuGGACGCCGCCGGCGAuuCCCCuuuCGCCGGCuuCuuCAGCuuCCCACAGuACuCCACGuCGCCuCGCACuGGCuGCAGCGCCGCCGCCAGCGCCGGGAGCuCCGGCAGuGCGAGCAGCGuCGuCAuGGACGACACGGCGGGCuCCGCGGAGAGCGGCCGGCAGuCGGCGGCCAuCGCCGACAuCGAGGuGACCAuGGuGGAAGGGCACGCGAGCCuCAAGGuGCuCGCGCGGCGGCGACCGAAGCAGCuGCuGAAGCuGGuCGuCGGGCuGCAGCAGCuGCGCAuCCCGCCGCuGCAuCuCAACGuGACCACCGuCGACGCCAuGGuCCuCuACuCCuuCAGCCuCAAGGuGGAGGAuGAuuCCAAGCuGGGCuCuGuGGAAGACAuuGCCACCGCuGuGCAuCAGAuCCuGGGCAGCAuuCAGCAGCAGGAGGCCGuCCuCuCCAuCAGC