Tfome Clone Information

TFome Information for pUT1675

TFome Name pUT1675
Template
Genbank Insert ID HQ858866
Protein Name OsbHLH47
Species Rice
TF Family bHLH
Gene ID LOC_Os03g08930
transcript
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: 3' Primer Name: R235DN
5' Primer Tm (º C): 56.2 3' Primer Tm (º C): 58.2
5' Primer Sequence: caccatggcgttagaggc 3' Primer Sequence: gctgatggagaggacggc
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Nucleotide Sequence: AuGGCGuuAGAGGCCGuGGuGuuuCCGCAGGGCCAuuuCGGCuACGGGuGCGGAAGGGACuCGCCGGCGuACGGGAuGCCGuGGuGCGACGuCCuGGCuGCuGCCGGCGGCGGCGGCGGCuuCGGuGAGuuuuACGGAGuGGACGAGuGGGAuGACCAGCuGCAGGuCGCCuCCGuGGAuGAGuGGGAGGuGGCAuCCAAGGAuAACuCGGAuGCuuCCACGGAGGGCAAGGCGGCGGCGGCGGAGCGGGCGGAGCCGGuGGCCGCCGGGAGGAGGAAACGGAGGCGGACGAAGGuCGuCAAGAACAAGGAGGAGAuCGAGuGCCAGCGGAuGACCCACAuuGCCGuCGAGCGCAACCGCCGCCGCCAGAuGAACGAGuACCuCGCCGuGCuCCGCuCCCuCAuGCCGGCGuCCuACuCGCAGAGGGGuGAuCAAGCAuCGAuCGuCGGAGGAGCAAuCAACuACGuGAAGGAGCuGGAGCAGCuACuGCAGuCGCuGGAAGuCCAAAAGAGCCuCAAGAACCGCAGCGGCGCCAuGGACGCCGCCGGCGAuuCCCCuuuCGCCGGCuuCuuCAGCuuCCCACAGuACuCCACGuCGCCuCGCACuGGCuGCAGCGCCGCCGCCAGCGCCGGGAGCuCCGGCAGuGCGAGCAGCGuCGuCAuGGACGACACGGCGGGCuCCGCGGAGAGCGGCCGGCAGuCGGCGGCCAuCGCCGACAuCGAGGuGACCAuGGuGGAAGGGCACGCGAGCCuCAAGGuGCuCGCGCGGCGGCGACCGAAGCAGCuGCuGAAGCuGGuCGuCGGGCuGCAGCAGCuGCGCAuCCCGCCGCuGCAuCuCAACGuGACCACCGuCGACGCCAuGGuCCuCuACuCCuuCAGCCuCAAGGuGGAGGAuGAuuCCAAGCuGGGCuCuGuGGAAGACAuuGCCACCGCuGuGCAuCAGAuCCuGGGCAGCAuuCAGCAGCAGGAGGCCGuCCuCuCCAuCAGC
TFome clones do not contain stop codon at 3'end.
translation: MALEAVVFPQGHFGYGCGRDSPAYGMPWCDVLAAAGGGGGFGEFYGVDEWDDQLQVASVDEWEVASKDNSDASTEGKAAAAERAEPVAAGRRKRRRTKVVKNKEEIECQRMTHIAVERNRRRQMNEYLAVLRSLMPASYSQRGDQASIVGGAINYVKELEQLLQSLEVQKSLKNRSGAMDAAGDSPFAGFFSFPQYSTSPRTGCSAAASAGSSGSASSVVMDDTAGSAESGRQSAAIADIEVTMVEGHASLKVLARRRPKQLLKLVVGLQQLRIPPLHLNVTTVDAMVLYSFSLKVEDDSKLGSVEDIATAVHQILGSIQQQEAVLSIS