Tfome Clone Information

TFome Information for pUT1676

TFome Name pUT1676
Template
Genbank Insert ID HQ858867
Protein Name OsbHLH65
Species Rice
TF Family bHLH
Gene ID LOC_Os03g58330
transcript
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: 3' Primer Name: R208DN
5' Primer Tm (º C): 64.8 3' Primer Tm (º C): 61.8
5' Primer Sequence: caccATGGCCGGGCAGCAG 3' Primer Sequence: GGAAGGAGTGTTGGGTTCGTGCTTC
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Nucleotide Sequence: AuGGCCGGGCAGCAGCCGCAGCAGCAGGGCCCACCGGAGGACGACuuuuuCGACCAGuuCuuCuCCCuGACCAGCuCCuuCCCuGGCGCCGCGCCGGGCGGCCGCGCCGCCGGuGACCAGCCCuuCuCCCuCGCGCuCAGCCuCGACGCCGCCGCGGCGGCCGAGGCGuCCGGGAGCGGGAAGAGGCuCGGGGuCGGCGAuGACGCCGAGGGuGGCGGCAGCAAGGCGGAuCGGGAGACCGuGCAGCuCACCGGACuCuuCCCGCCGGuGuuCGGCGGCGGCGGCGuGCAGCCGCCGAACCuCCGCCCCACCCCGCCuACCCAGGuGuuCCACCCGCAGCAGuCGAAGCAGGGCGGAGCAGCCGuCGGGCCGCAGCCGCCGGCGCCGAGGCCGAAGGuGCGAGCGCGGCGuGGGCAGGCGACCGACCCCCACAGCAuCGCGGAGAGGCuAAGAAGAGAGAGAAuAGCAGAAAGGAuGAGGGCCCuACAGGAAuuGGuCCCCAAuACAAACAAGACAGAuAGGGCAGCuAuGCuAGAuGAGAuCCuuGAuuAuGuGAAAuuCCuGAGGCuGCAAGuAAAGGuuuuAAGCAuuAGCAGGCuGGGuGGCGCGGGuGCuGuuGCACAGCuGGuuGCuGAuAuuCCACuuuCAGuuAAGGGGGAAGCAAGCGAuAGuGGGGGCAACCAACAGAuuuGGGAAAAGuGGuCAACGGAuGGCACAGAAAGACAGGuAGCGAAGCuGAuGGAAGAAGACAuCGGGGCAGCGAuGCAAuuuCuCCAAuCCAAAGCACuCuGCAuGAuGCCAAuCuCGCuCGCCAuGGCAAuCuAuGACACACAACAAACACAGGAuGGACAACCAGuGAAGCACGAACCCAACACuCCuuCC
TFome clones do not contain stop codon at 3'end.
translation: MAGQQPQQQGPPEDDFFDQFFSLTSSFPGAAPGGRAAGDQPFSLALSLDAAAAAEASGSGKRLGVGDDAEGGGSKADRETVQLTGLFPPVFGGGGVQPPNLRPTPPTQVFHPQQSKQGGAAVGPQPPAPRPKVRARRGQATDPHSIAERLRRERIAERMRALQELVPNTNKTDRAAMLDEILDYVKFLRLQVKVLSISRLGGAGAVAQLVADIPLSVKGEASDSGGNQQIWEKWSTDGTERQVAKLMEEDIGAAMQFLQSKALCMMPISLAMAIYDTQQTQDGQPVKHEPNTPS