Tfome Clone Information

TFome Information for pUT1684

TFome Name pUT1684
Template
Genbank Insert ID HQ858871
Protein Name OsbHLH148
Species Rice
TF Family bHLH
Gene ID LOC_Os03g53020
transcript
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: 3' Primer Name: R217DN
5' Primer Tm (º C): 59.4 3' Primer Tm (º C): 58.3
5' Primer Sequence: caccatgcaaatggagtcgtactacg 3' Primer Sequence: aaacacattttgcacatgagatgtctgtg
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Nucleotide Sequence: AuGCAAAuGGAGuCGuACuACGGCGCGuuCCACGCCGACGAGGCCGCGuuCuuCuuCCCCCACCACGuGCCGGCGuCGCCGGAGCuGCCGuuCGGCCuCAuuGCGuCGCCGGAGCCGGAGCCGGAGCCGGAGCAGGCGGCGGCGGAGGCGAGGCAGAGCGCGuuCCAGGAGuACGGCGGCGCGGuGCACGCCGGAGCGCCGGCGGCGGCGGGGGCuGuCACCACCGGuGGGACGAACAuCCACCGGAGGGuGAuGGACGuGCuGGGCAGGAuGGGCGGCGGCGGCGGCGGGGGGGAGAAGGGGGAGGGuGAGGAGAuGGAGGAGGAGGAGGAGGuGCCGCAGCGGCGGCGGCGCGGGCAGGGCGCCGACGuGGAGAGCAGCCGCGGuuuCCGCCACAuGAuGCGCGAGCGCCAGCGCCGCGAGAAGCuCAGCCAGAGCuACGCCGACCuCuACGCCAuGGuCuCCuCuCGCuCCGAGGGGGACAAGAACuCGAuCGuGCAGuCGGCGGCGAuCuACAuCCACGAGCuGAAGGuCGCGAGGGACCAGCuGCAGAGGAGGAACGAGGAGCuGAAGGCCCAGAuCAuGGGCCACGACGAGCAGCAGCCGuGCGuCACGGuGCAGuuCGAGGuCGACGAGCCGuCGuCGuCGAuCGACuCCAuGAuCGCCGCGCuCCGGCGGCuCAAGAGCAuGAGCGuCAAGGCGCGCGGGAuCCGGuCGAGCAuGuCCGGGAACAGGCuGuGGACGGAGAuGAACGuCGAGACCACGAuuGCGGCuuGuGAAGuGGAAAAGGCAGuGGAAGAGGCuCuCAAGGAAGuAGAGAGGAACCAGCCuGACAGCGAuGCCCCAuuuCCuGGAAGCAAAGGCuGGACACAGACAuCuCAuGuGCAAAAuGuGuuu
TFome clones do not contain stop codon at 3'end.
translation: MQMESYYGAFHADEAAFFFPHHVPASPELPFGLIASPEPEPEPEQAAAEARQSAFQEYGGAVHAGAPAAAGAVTTGGTNIHRRVMDVLGRMGGGGGGGEKGEGEEMEEEEEVPQRRRRGQGADVESSRGFRHMMRERQRREKLSQSYADLYAMVSSRSEGDKNSIVQSAAIYIHELKVARDQLQRRNEELKAQIMGHDEQQPCVTVQFEVDEPSSSIDSMIAALRRLKSMSVKARGIRSSMSGNRLWTEMNVETTIAACEVEKAVEEALKEVERNQPDSDAPFPGSKGWTQTSHVQNVF