| TFome pUT1752 | |
|---|---|
| Template | |
| Genbank Insert ID | HQ858875 |
| Protein Name | |
| Species | Oryza sativa |
| TF Family | |
| Gene id | LOC_Os05g09020 |
| transcript | |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1752
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | |
| Temperature (ºC) | 64.2 |
| Sequence
|
caccATGGCGGCTTCCGTAGGAC |
3' Primer
| Name | R207DN |
| Temperature (ºC) | 67.7 |
| Sequence
|
GAAGAGCAGCGAGCCGCCTGCCGAATAG |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MAASVGLNPEAFFFSNSYSYSSSPFMASYTPEFSAAAIDANLFSGELDFDCSLPAPAQEYPENENTMMRYESEEKMRARVNGRIGFRTRSEVEILDDGFKWRKYGKKAVKNSPNPRNYYRCSTEGCNVKKRVERDREDHRYVITTYDGVHNHASPAAAAAALQYAAAAGDYYSPPLSSAVSPPAAYSAGGSLLF
TFome clones do not contain stop codon at 3'end.
CDS
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AuGGCGGCuuCCGuAGGACuGAACCCuGAAGCuuuCuuCuuCAGCAACuCCuACuCCuACuCCuCAuCCCCuuuCAuGGCCAGCuACACGCCGGAGuuCuCGGCCGCCGCuAuCGACGCCAACuuAuuCuCCGGCGAGCuCGAuuuCGACuGCuCuCuCCCAGCuCCGGCCCAGGAGuACCCGGAAAAuGAAAACACuAuGAuGAGGuACGAGAGCGAGGAGAAGAuGAGGGCGAGGGuGAACGGGAGGAuCGGGuuCAGGACGAGGuCGGAGGuGGAGAuuCuuGAuGAuGGCuuCAAGuGGAGGAAGuACGGCAAGAAAGCuGuCAAGAACAGCCCAAAuCCAAGAAACuACuACCGGuGCuCGACGGAGGGGuGCAACGuGAAGAAGCGAGuGGAGAGAGACCGGGAGGACCACCGCuACGuCAuCACCACCuACGACGGCGuCCACAACCACGCGAGCCCCGCCGCCGCCGCCGCGGCGCuGCAGuACGCCGCCGCCGCCGGCGACuACuACAGCCCGCCGCuCAGCAGCGCCGuCuCGCCGCCGGCCGCCuAuuCGGCAGGCGGCuCGCuGCuCuuC
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