Tfome Clone Information

TFome Information for pUT1752

TFome Name pUT1752
Template
Genbank Insert ID HQ858875
Protein Name OsWRKY49
Species Rice
TF Family WRKY
Gene ID LOC_Os05g09020
transcript
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: 3' Primer Name: R207DN
5' Primer Tm (º C): 64.2 3' Primer Tm (º C): 67.7
5' Primer Sequence: caccATGGCGGCTTCCGTAGGAC 3' Primer Sequence: GAAGAGCAGCGAGCCGCCTGCCGAATAG
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Nucleotide Sequence: AuGGCGGCuuCCGuAGGACuGAACCCuGAAGCuuuCuuCuuCAGCAACuCCuACuCCuACuCCuCAuCCCCuuuCAuGGCCAGCuACACGCCGGAGuuCuCGGCCGCCGCuAuCGACGCCAACuuAuuCuCCGGCGAGCuCGAuuuCGACuGCuCuCuCCCAGCuCCGGCCCAGGAGuACCCGGAAAAuGAAAACACuAuGAuGAGGuACGAGAGCGAGGAGAAGAuGAGGGCGAGGGuGAACGGGAGGAuCGGGuuCAGGACGAGGuCGGAGGuGGAGAuuCuuGAuGAuGGCuuCAAGuGGAGGAAGuACGGCAAGAAAGCuGuCAAGAACAGCCCAAAuCCAAGAAACuACuACCGGuGCuCGACGGAGGGGuGCAACGuGAAGAAGCGAGuGGAGAGAGACCGGGAGGACCACCGCuACGuCAuCACCACCuACGACGGCGuCCACAACCACGCGAGCCCCGCCGCCGCCGCCGCGGCGCuGCAGuACGCCGCCGCCGCCGGCGACuACuACAGCCCGCCGCuCAGCAGCGCCGuCuCGCCGCCGGCCGCCuAuuCGGCAGGCGGCuCGCuGCuCuuC
TFome clones do not contain stop codon at 3'end.
translation: MAASVGLNPEAFFFSNSYSYSSSPFMASYTPEFSAAAIDANLFSGELDFDCSLPAPAQEYPENENTMMRYESEEKMRARVNGRIGFRTRSEVEILDDGFKWRKYGKKAVKNSPNPRNYYRCSTEGCNVKKRVERDREDHRYVITTYDGVHNHASPAAAAAALQYAAAAGDYYSPPLSSAVSPPAAYSAGGSLLF