TFome clone pUT1753

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	NLP UP
Temperature (ºC)	64.1
Sequence Copy to clipboard	caccATGGACGCCGCCGT

3' Primer

Name	NLP DN
Temperature (ºC)
Sequence Copy to clipboard	ATCCATATCCATGACTGAACTTTTGGCC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MDAAVSTLTALAIFASTVEHAAYRSVQGYRVVGRKGGGWVRWERWVERQFVLSLSFPRCVEVALPAAAPRILPAGWRSRPVFREGQTVDTWRCIVAFDSVAAVAPSSPPPPVLSPFVNPQLQYLSNLYNDLLMVFRFREEKTVPQLVNSKQPIRCGEQGKTSDLGDASESDSDGDCQSGKELAPPVQKHTRANRKHIDSITLVDIAQYFHLPIRDASKTLKIGVSILKRKCRQYGIPRWPHRKIKSLDSLIHDLEFVLAREDEDEEEEKQLQKDRLAAAINALTKRKSMLESEKETIQQKPAMDLMAETKLFREDVFKRRYRAKSSVMDMD

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACGCCGCCGTCTCCACCCTCACCGCTCTCGCCATCTTCGCGAGCACCGTCGAGCACGCCGCCTACAGGAGCGTGCAGGGGTATAGGGTGGTCGGGAGGAAAGGCGGCGGGTGGGTCCGTTGGGAGAGGTGGGTGGAGCGGCAGTTCGTCCTCTCCCTCTCCTTCCCACGGTGCGTCGAGGTTGCCCTGCCCGCCGCCGCGCCGCGGATACTGCCAGCGGGGTGGCGCAGCCGGCCGGTGTTCCGCGAGGGACAGACCGTCGACACTTGGCGTTGCATTGTGGCATTCGACTCCGTCGCCGCCGTCGCTCCGTCCTCCCCGCCGCCGCCCGTGCTCTCCCCCTTCGTGAACCCACAGCTGCAGTATCTGTCTAATCTGTACAATGACCTGCTGATGGTGTTTCGGTTTCGAGAAGAGAAAACGGTCCCACAACTCGTTAATTCAAAGCAACCCATCCGTTGTGGTGAACAGGGGAAAACTTCTGATTTGGGTGATGCATCGGAGTCCGATTCGGATGGGGATTGTCAATCTGGCAAAGAACTTGCACCACCAGTTCAGAAGCATACAAGAGCCAACCGGAAGCATATAGATAGCATTACTTTAGTTGATATAGCTCAGTACTTCCATCTTCCAATCCGAGATGCATCAAAGACACTCAAGATTGGGGTCAGCATATTGAAGAGGAAATGCCGACAGTATGGGATACCTCGTTGGCCTCACCGGAAGATCAAGTCACTTGACTCTCTCATTCATGACCTTGAGTTCGTGCTAGCAAGAGAGGATGAGGATGAGGAGGAGGAGAAGCAGCTGCAGAAGGACAGGCTGGCTGCTGCGATAAACGCCCTCACAAAACGGAAGAGCATGCTGGAGAGCGAGAAGGAAACCATACAGCAGAAACCGGCCATGGACCTGATGGCTGAAACCAAGCTATTCAGGGAAGATGTTTTCAAGAGGAGATATAGGGCCAAAAGTTCAGTCATGGATATGGAT