| TFome pUT1755 | |
|---|---|
| Template | |
| Genbank Insert ID | HQ858877 |
| Protein Name | |
| Species | Oryza sativa |
| TF Family | |
| Gene id | LOC_Os05g40280 |
| transcript | |
| Vector | pENTR/SD/D-TOPO |
| Note | in the genomic DNA, the nucleotide is C at the 470th allele of the ORF, while it is changing to G at the same position in the entry clone. |
| Request Information | Deposited to ABRC |
TFome clone pUT1755
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | |
| Temperature (ºC) | 72.3 |
| Sequence
|
3' Primer
| Name | XL05g04814R |
| Temperature (ºC) | 60.8 |
| Sequence
|
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MAAEAGSAAAGAAYEEERRKRVLENLKHLEDLGIKKMSKSLLEAARLQKSTRASPKPRKKFEVGATEVRRSSRARNSVSYKENFDELNSFLCRRRGSRIRSTEQGRDYTGRVASYEQQQRAFKKAERLQNSLDPENPSFVKTMVRSHVSSCFWLGLRTRFCKLHLPPKEYKMVLEDEEGGEFDSVYIGNRTGLSGGWRGFAMHHNLEDGDSLVFELAEPDRFKIYIIKAVDEDANESEPADEEAIGDKDTSTEDAAEQDDSPNAEPLKGTKRRKLRGRR
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGGCGGAGGCCGGCAGCGCGGCCGCCGGCGCCGCCTACGAGGAGGAGCGCCGGAAGCGGGTCCTCGAGAACCTCAAGCACCTCGAGGATCTGGGCATAAAGAAGATGTCCAAGAGCTTGCTGGAGGCGGCGAGATTGCAGAAGAGCACTCGTGCCAGCCCCAAGCCGCGGAAGAAGTTTGAGGTTGGGGCCACTGAAGTGAGGCGTTCATCGAGGGCGCGCAACTCGGTTTCGTACAAAGAGAATTTTGATGAACTCAACAGTTTTCTATGCCGTAGAAGGGGCAGTAGGATTAGGAGCACAGAGCAGGGGAGGGATTACACCGGAAGAGTTGCCTCTTATGAACAGCAACAACGTGCTTTCAAAAAAGCTGAGAGACTTCAAAATAGTCTAGACCCTGAGAACCCGTCGTTTGTTAAGACCATGGTTCGATCACATGTGTCCAGCTGCTTTTGGCTTGGTCTTCGAACTAGATTCTGCAAATTGCATCTGCCTCCCAAAGAGTATAAGATGGTCTTGGAGGATGAAGAGGGTGGTGAATTTGATTCTGTCTACATTGGGAACCGTACAGGCCTAAGTGGTGGATGGAGAGGTTTTGCGATGCACCACAACTTGGAAGATGGTGATTCATTGGTCTTTGAATTGGCTGAACCAGACAGATTTAAGATCTACATCATAAAAGCTGTTGATGAGGATGCAAATGAGTCTGAGCCTGCTGATGAGGAAGCTATTGGGGACAAGGACACCAGTACAGAGGATGCAGCTGAGCAGGATGACTCACCTAATGCTGAACCTTTAAAAGGAACTAAGAGGAGAAAACTCCGTGGACGCCGG
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