| TFome pUT1758 | |
|---|---|
| Template | EE186043 |
| Genbank Insert ID | HQ858772 |
| Protein Name | ZmWRKY34 |
| Species | Zea mays |
| TF Family | WRKY |
| Gene id | GRMZM2G057116 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.C611T p.A204V. Not in functional domain. Not in template FlcDNA. |
| Request Information | Deposited to ABRC |
TFome clone pUT1758
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C107UP |
| Temperature (ºC) | 61.4 |
| Sequence
|
caccATGGCGACTTCGCTGG |
3' Primer
| Name | C107DN |
| Temperature (ºC) | |
| Sequence
|
GAA GAC TAG GGA GCC CGA GTA GGA G |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MATSLGLNPEDLFTSYSSSYYSSPPFMSDYAASFTPAAGDSTAFSSELDDLHHFDYSPAPIVTAAGAGAGGGDRNEKMMWCEGGGDERRLRSNGRIGFRTRSEVEILDDGFKWRKYGKKAVKNSPNPRNYYRCSSEGCGVKKRVERDRDDPRYVITTYDGVHNHASPGAAAIIVPYGSGGGNSGFYSPPHSGSPSATSYSGSLVF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGACTTCGCTGGGACTGAACCCTGAAGATCTCTTCACTTCGTACTCGTCTTCCTACTACTCCTCGCCGCCGTTCATGTCCGACTACGCGGCGAGCTTCACGCCGGCGGCCGGGGACTCCACGGCCTTCTCCTCGGAGCTCGACGACCTTCACCACTTCGACTACTCACCGGCGCCGATCGTCACTGCTGCCGGAGCCGGGGCTGGGGGCGGCGATCGCAACGAGAAGATGATGTGGTGTGAGGGTGGTGGTGACGAGAGAAGACTCAGAAGCAACGGAAGGATCGGGTTCAGAACGAGGTCAGAGGTGGAGATCTTGGACGACGGATTCAAGTGGAGGAAGTACGGGAAGAAGGCCGTCAAGAACAGCCCAAATCCAAGGAACTACTACCGCTGCTCGTCGGAGGGCTGCGGCGTGAAGAAGCGGGTGGAGAGGGACCGCGACGACCCCCGCTACGTCATCACCACCTACGACGGCGTCCACAACCACGCCAGCCCCGGAGCCGCTGCTATCATCGTCCCGTACGGCAGCGGCGGCGGCAATAGCGGCTTCTACAGCCCGCCGCACAGCGGCTCCCCGTCGGCCACCTCCTACTCGGGCTCCCTAGTCTTC
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