| TFome pUT1765 | |
|---|---|
| Template | DR963059 |
| Genbank Insert ID | HQ858779 |
| Protein Name | ZmMYB149 |
| Species | Zea mays |
| TF Family | MYB |
| Gene id | GRMZM2G127490 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT1765
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B178UP |
| Temperature (ºC) | 58.5 |
| Sequence
|
caccATGGGGAGGCATTCTTG |
3' Primer
| Name | B178DN |
| Temperature (ºC) | |
| Sequence
|
GATATTCTCAAAAGACAGTTGCATTCTTTG |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MGRHSCCYKQKLRKGLWSPEEDEKLMNHITKHGHGCWSSIPKLAGLQRCGKSCRLRWINYLRPDLKRGAFSQDEEDLIIELHAVLGNRWSQIAAQLPGRTDNEIKNLWNSCIKKKLRQKGIDPNTHKPLTEADRSGAAPTISTERTSGSSEINPSSAGALGNLSHLLSETAQSSMLMPVYDKNRAETPNLARPKVPPKELFLEQLTAGHESPSTCRSSGQTLYFPFQQPLGYNSESGSGDGANMNSLWFNQSDFNCSTISTVMPPASPSALSTSMGLNLPPDNSRHGGTGIGSTAVDSFYWDGTNPSSSSSTGSRGSNSMGFEPQSTSSILENSVFQWTDIGQEKDTRAHLVEELKWPDLLHGTFAETTTAMQNQSQTLYDDVIKAESQFSMEGICASWFQNQQPQQQLQATPDMYDKDLQRMQLSFENI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGGAGGCATTCTTGCTGCTACAAGCAAAAGTTGAGAAAGGGGCTCTGGTCCCCCGAGGAGGATGAGAAACTCATGAACCACATAACAAAGCATGGCCATGGCTGCTGGAGTTCTATTCCCAAACTCGCTGGGCTCCAAAGGTGCGGAAAGAGTTGTAGGCTGAGGTGGATAAACTATCTGAGGCCAGACCTCAAGAGAGGGGCATTCTCCCAAGACGAAGAAGACCTCATCATTGAGCTGCACGCTGTCCTTGGAAACAGGTGGTCTCAAATTGCAGCTCAGCTGCCAGGGAGGACTGATAATGAGATCAAGAATCTATGGAACTCGTGCATCAAGAAGAAGCTCAGGCAGAAAGGCATTGATCCCAACACCCACAAGCCCCTTACTGAGGCTGATCGCAGCGGAGCAGCTCCCACCATCAGCACCGAGAGAACCTCAGGGTCCAGCGAAATCAACCCATCAAGCGCTGGTGCTCTGGGAAACTTGAGCCACCTCCTCAGCGAGACGGCTCAATCATCAATGCTGATGCCCGTGTATGACAAGAATCGCGCTGAAACTCCAAACTTGGCACGCCCTAAGGTGCCACCAAAGGAGTTGTTCCTGGAGCAGCTTACTGCTGGACACGAGAGTCCATCAACCTGCCGCTCATCAGGCCAAACTCTCTATTTCCCTTTCCAACAGCCTTTAGGCTACAACAGTGAGAGTGGCAGTGGCGATGGGGCGAATATGAATTCACTCTGGTTCAACCAGAGTGACTTCAACTGCAGCACAATTTCTACAGTGATGCCGCCAGCTTCACCATCGGCCCTCTCTACATCAATGGGGCTGAATCTTCCACCTGATAATTCTCGACATGGAGGAACTGGCATTGGAAGTACTGCAGTAGATTCATTCTACTGGGATGGTACTAATCCTAGCAGCAGTAGCAGTACTGGGAGCAGAGGAAGCAACAGTATGGGATTTGAACCACAGAGCACGAGCTCAATTCTGGAGAACAGTGTCTTCCAATGGACAGACATCGGACAAGAAAAAGATACAAGAGCTCACCTGGTAGAGGAACTTAAGTGGCCTGACTTGCTCCATGGAACCTTTGCCGAGACGACCACAGCCATGCAGAACCAGAGCCAGACGCTCTATGATGACGTGATCAAGGCAGAGAGCCAATTCAGCATGGAAGGGATATGTGCTTCTTGGTTCCAGAATCAGCAGCCACAGCAACAGCTTCAGGCAACACCAGACATGTATGACAAGGACTTGCAAAGAATGCAACTGTCTTTTGAGAATATC
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