| TFome pUT1770 | |
|---|---|
| Template | EE011258 |
| Genbank Insert ID | HQ858784 |
| Protein Name | ZmHB25 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G178102 |
| transcript | T01 |
| Vector | pENTR/SD/D-TOPO |
| Note | c.Start+297 Supported by FlcDNA and RNA-seq. Does not effect functional domains. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT1770
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C92UP |
| Temperature (ºC) | 70.8 |
| Sequence
|
caccATGGCGGCGGTGGCGAGCAG |
3' Primer
| Name | C92DN |
| Temperature (ºC) | |
| Sequence
|
GATGAAGGTCCAGTTGGCGAGCA |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MAAVASRERLSPGAAPQVDAGKYVRYTPEQVEALERVYSECPKPSSLRRQHLIRDCPILRNIEPKQIKVWFQNRRCREKQRKESSRLQTVNRKLSAMNKLLMEENDRLQKQVSRLVFDNGYMKNRLHSPSVATTDTSCESVVTSGQHNQQQNPAVLHPPQRDANNPAGLLAIAEETLAEFMSKATGTAVNWVQMVGMKPGPDSVGIIAVSHNCSGVAARACGLVSLEPTKVAEILKDRASWYRDCRHVDVLHVIPTGNGGTIELIYMQTYALTTLAEPRDFWTLRYTSGLDDGSLVICERSLTQSTGGPSGPNTPNFIRAEVLPSGYLIRPCDGGGSMIYIVDHVNLNACSVPEVLRPLYESPKILAQKMTAAALRHIRQIAHESSGEMPYGAGRQPAVLRTFSQRLSRGFNDAVSGFPDDGWSCLLNTDGAEDITVTINSSPNKLVGSHVSASPLFSAMGGGIMCAKASMLLQNVPPALLVRFLREHRSEWADPGIDAYSVASLRANPYTVPGLRAGGFMGNQVILPLARTLEHEESLEVIRLEGHGFSHEEVLMSRDMFLLQLCSGVDEDAPGACAQLVFAPIDESFADDAPLLPSGFRVIPLDAKTDVPTATTRTLDLASALEVGSGGSGMRASCDGSGTCATRSVLTIAFQFSFENHLRESVAAMAKQYVRGVMASVQRVAMAIAPSRLGSRIELKHPPGSPEALALATWIGRSYRAHTGTEIRWSDTEGADSPLMLFWKHSDAILCCSLKPPAFTLKFANSAGFDILETTMVNIQDLPLEAVLDEEGQKALSAQLPAIMQQGLAYLPGGVCRSSMGRQASYEQAVAWKVVGDDGAPQCLALMLANWTFI
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGGCGGTGGCGAGCAGGGAGAGGCTCTCCCCGGGCGCCGCGCCGCAGGTGGACGCGGGCAAGTACGTGCGGTACACGCCGGAGCAGGTGGAGGCACTGGAGCGCGTCTACAGCGAGTGCCCCAAGCCCAGCTCGCTGCGCCGGCAGCACCTCATCAGGGACTGCCCCATCCTCCGCAACATCGAGCCCAAGCAGATCAAGGTCTGGTTCCAGAACCGCAGATGCCGGGAGAAGCAGCGCAAGGAGTCCTCTCGTCTACAAACCGTGAACCGGAAGCTGAGTGCGATGAACAAGCTGTTGATGGAGGAGAATGACCGGCTGCAGAAGCAGGTTTCCCGTCTCGTCTTTGACAACGGGTATATGAAGAATCGACTCCACAGTCCTTCTGTAGCCACCACCGACACAAGCTGTGAGTCTGTGGTGACAAGTGGTCAGCACAACCAACAGCAAAACCCAGCAGTTCTGCATCCTCCACAAAGGGATGCGAACAACCCAGCAGGTCTACTCGCTATTGCTGAGGAGACATTGGCAGAGTTCATGTCCAAGGCGACTGGAACTGCTGTCAACTGGGTGCAAATGGTTGGGATGAAGCCTGGTCCGGATTCCGTTGGAATCATCGCTGTTTCGCACAACTGCAGCGGCGTAGCAGCACGAGCTTGCGGCCTTGTGAGCCTTGAGCCCACAAAGGTTGCTGAGATCCTTAAGGATCGTGCATCGTGGTATCGCGATTGTCGGCATGTTGATGTCCTCCATGTTATCCCTACGGGTAACGGTGGAACGATTGAGCTGATCTATATGCAGACTTATGCACTGACAACTCTCGCGGAACCACGAGACTTTTGGACGCTCCGATACACTAGTGGTCTTGACGATGGCAGTCTTGTGATCTGCGAAAGGTCATTGACTCAGTCCACTGGAGGTCCTTCTGGACCTAACACTCCAAATTTTATCAGAGCTGAGGTGCTTCCTAGTGGTTATCTGATTCGACCCTGTGATGGGGGAGGTTCCATGATTTACATTGTGGATCATGTTAATCTGAATGCTTGTAGTGTGCCTGAGGTTCTTCGGCCACTCTATGAATCCCCTAAGATACTGGCACAGAAGATGACTGCGGCGGCGTTGCGACACATTAGGCAAATTGCACATGAGTCAAGTGGTGAAATGCCCTATGGTGCTGGGCGACAGCCGGCTGTTCTCAGAACTTTCAGTCAAAGGCTCAGCAGAGGCTTCAATGATGCTGTGAGTGGCTTTCCAGATGATGGCTGGTCTTGTTTGTTGAACACCGATGGTGCTGAGGATATTACAGTTACAATCAACTCATCTCCAAATAAACTTGTTGGGTCTCATGTCAGCGCTTCCCCATTATTTTCTGCTATGGGGGGTGGAATCATGTGCGCAAAAGCATCGATGCTGCTGCAGAATGTGCCACCTGCTCTACTTGTGCGATTTTTGAGGGAGCATCGGTCTGAATGGGCTGATCCTGGCATCGATGCTTATTCTGTCGCCTCTTTGAGGGCCAACCCATACACTGTTCCAGGTTTAAGGGCTGGTGGGTTTATGGGCAACCAGGTTATACTGCCCCTTGCACGCACCTTGGAGCATGAAGAGTCCTTGGAGGTTATTAGGCTTGAAGGACATGGCTTTAGCCATGAGGAGGTGCTTATGTCCCGGGATATGTTTCTTCTACAGCTGTGCAGTGGCGTCGACGAGGATGCACCTGGTGCGTGTGCACAGCTTGTCTTTGCTCCTATTGATGAATCTTTTGCCGACGATGCACCGTTGCTGCCCTCAGGCTTCCGTGTGATACCGCTTGATGCTAAGACGGATGTGCCAACGGCCACCACACGCACGCTTGACCTTGCCTCTGCGCTCGAGGTTGGATCAGGTGGAAGCGGCATGCGCGCTTCATGCGATGGTTCCGGCACGTGCGCCACGAGATCGGTCCTGACCATCGCCTTCCAGTTCTCGTTCGAGAACCACCTTCGCGAGAGCGTGGCGGCAATGGCCAAGCAGTACGTCAGGGGCGTGATGGCCTCCGTGCAGAGGGTGGCCATGGCGATCGCTCCTTCTCGCCTCGGTTCGCGTATCGAACTGAAGCATCCGCCGGGCTCTCCCGAGGCACTTGCACTAGCTACGTGGATTGGCAGGAGCTACAGGGCGCACACTGGAACGGAGATCCGCTGGTCGGACACCGAAGGCGCGGACTCTCCACTGATGCTGTTCTGGAAGCACAGCGACGCGATACTCTGCTGCTCTCTGAAGCCGCCGGCTTTCACGCTCAAGTTCGCCAACAGCGCCGGCTTCGACATCCTGGAGACGACGATGGTGAACATCCAGGACCTGCCGCTGGAGGCGGTCCTCGACGAGGAGGGCCAGAAGGCCCTGTCCGCGCAGCTCCCCGCGATCATGCAGCAGGGCCTGGCGTACCTCCCCGGTGGCGTGTGCAGGTCGAGCATGGGGCGGCAGGCGTCGTACGAGCAGGCAGTGGCGTGGAAGGTGGTGGGTGACGACGGCGCCCCGCAGTGCCTGGCGCTCATGCTCGCCAACTGGACCTTCATC
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