TFome clone pUT1802

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B200UP
Temperature (ºC)	61.8
Sequence Copy to clipboard	caccATGGATCAACTTGCCCTGCT

3' Primer

Name	B200DN
Temperature (ºC)
Sequence Copy to clipboard	TGTTCCCAAGTATTCCATTAAATCATCATC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

Copy to clipboard

MDQLALLATELGDSSDFEVEGIQNLTENDVSDEEIEPEDLARRMWKDRVRLRRIKERQHKLALQQAELQKLRPKPISDQAMRKKMSRAHDGILKYMLKLMQVCNARGFVYGIIPDKGKPVSGASDNIRAWWKEKVKFDKNGPAAIEKYESDNLVSATAQSGGTKSQHSLMDLQDATLGSLLSSLMQHCDPPQRKYPLEKGASPPWWPSGKEEWWTALGLPSGQVPPYKKPHDLKKVWKAGVLTGVIKHMSPNFDKIRNHVRKSKCLQDKMTAKESLIWLGVLQREESLVHRIDNGVSEITQRSMPEERIADTNSSSNEYDVYGFEDAPVSTSSKDDEQDLSPVAQSAVEHVPKRGRERAYNKRPNQIVPGKAKEPPKRKRTRHSSTVTEPDAHRVVDAPENSRNLIPDMNRLDQVEIPGTSNQIVTFDHGGTTTEALQHRGDAQVQVHPPGAEVNSFYSAPTANPTPISIYMGDQPIPYQNNDSTRPRSENSFPVDAHTGLNNLPSSYQNLPPKQSLPLPMMDHHVVPMGIRAPTDSIPYGDHILGGGNSTSVPGDMQQLIDFPFYAEQDKFVGSSFEGLPLDYISISSPIPDIDDLLHDDDLMEYLGT

TFome clones do not contain stop codon at 3'end.

CDS

Copy to clipboard

ATGGATCAACTTGCCCTGCTTGCGACGGAGCTGGGTGACTCGTCAGACTTTGAGGTGGAGGGCATCCAGAACCTCACTGAGAACGATGTCAGTGACGAGGAGATTGAGCCCGAGGACCTGGCGCGGCGAATGTGGAAGGACAGAGTCAGGCTGAGGAGGATCAAGGAGCGGCAGCACAAGCTTGCCCTGCAGCAGGCAGAGCTGCAGAAGTTGAGGCCCAAGCCCATATCCGACCAGGCCATGCGCAAGAAGATGTCAAGGGCACACGACGGGATCCTCAAGTATATGCTCAAGCTCATGCAGGTGTGCAATGCACGCGGGTTCGTTTATGGGATCATCCCGGACAAGGGGAAGCCTGTCAGTGGCGCTTCCGATAATATCAGAGCTTGGTGGAAGGAGAAGGTGAAGTTTGATAAGAACGGGCCTGCGGCGATTGAGAAATACGAGTCTGACAACTTAGTATCCGCTACTGCCCAGAGTGGTGGAACTAAGAGCCAGCACAGCTTGATGGATCTCCAAGATGCCACTCTTGGTTCACTGCTTTCCTCATTGATGCAGCATTGTGATCCACCGCAGCGCAAGTACCCATTGGAGAAGGGTGCTTCACCCCCGTGGTGGCCTTCAGGGAAGGAGGAATGGTGGACCGCCCTGGGTCTTCCAAGCGGCCAAGTTCCTCCTTACAAGAAACCACATGATCTTAAGAAGGTCTGGAAGGCTGGTGTGCTTACAGGTGTAATCAAGCACATGTCTCCCAATTTTGATAAGATAAGGAATCATGTACGGAAATCAAAGTGTTTGCAGGATAAAATGACTGCAAAAGAAAGCCTGATTTGGCTGGGAGTTCTACAGAGAGAAGAAAGCCTTGTTCACAGAATCGACAATGGCGTATCGGAGATTACTCAGCGCAGTATGCCAGAGGAGAGGATTGCAGACACAAATAGCAGCAGTAATGAGTATGATGTGTATGGTTTTGAGGATGCTCCTGTTTCTACATCATCTAAAGATGATGAACAAGATCTGTCTCCAGTTGCACAATCTGCTGTGGAGCATGTTCCGAAAAGAGGAAGGGAAAGAGCTTACAATAAACGCCCTAATCAAATCGTTCCTGGTAAGGCAAAAGAACCACCAAAGAGAAAAAGAACACGCCACAGCTCCACTGTTACCGAGCCAGATGCACACAGAGTTGTTGATGCACCAGAAAACTCAAGAAATTTGATCCCTGATATGAATAGATTGGATCAAGTAGAAATCCCAGGCACGTCAAACCAGATTGTCACCTTTGACCATGGGGGCACCACAACTGAAGCTTTACAACACAGAGGAGATGCTCAAGTACAGGTCCATCCTCCTGGTGCTGAGGTTAATAGCTTCTATAGTGCTCCAACTGCAAACCCTACTCCTATAAGCATATATATGGGTGACCAGCCTATACCTTATCAAAATAATGACAGCACAAGGCCGAGATCTGAAAATAGTTTTCCTGTAGATGCTCATACTGGTTTAAATAATCTTCCCAGCAGTTATCAGAACTTACCTCCGAAACAATCACTACCACTTCCCATGATGGATCATCATGTGGTTCCCATGGGTATCAGGGCACCTACTGATAGCATTCCTTACGGTGATCATATACTAGGTGGTGGAAATTCAACTTCTGTTCCTGGAGATATGCAGCAACTCATAGATTTTCCTTTCTATGCTGAACAAGATAAGTTTGTTGGCAGTTCCTTTGAGGGGTTGCCTTTAGACTATATCAGTATCAGTAGTCCAATCCCAGATATTGATGACTTGCTGCATGATGATGATTTAATGGAATACTTGGGAACA