Tfome Clone Information

TFome Information for pUT1858

TFome Name pUT1858
Template
Genbank Insert ID HQ858878
Protein Name OsbHLH24
Species Rice
TF Family bHLH
Gene ID LOC_Os01g70310
transcript
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: 3' Primer Name: R226DN
5' Primer Tm (º C): 62.8 3' Primer Tm (º C): 62.2
5' Primer Sequence: caccatggacgaggcggagg 3' Primer Sequence: cattgcgttctgaaggccagcagag
PCR Condition: Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Nucleotide Sequence: AuGGACGAGGCGGAGGCGGCGGCGGCGGCGGCGAAGAuGGACGAGCuGGCGGGAGGAGGAGGAGGAGGCGGCGGCGACuGGuCGuACCuCGCGGCGGACGCGCuGGCGGCGGCGuCGuuCACGGCGuuCCCGuuCCACCACCACCACCACCACCACCACCGCGACGuGCuCuCGGCGuCGACCCCGuCGuCGCuGCuCCuCAACAuGGACGCGGCCACCGCCGCGGCCAuGuuCGACuuCCAGGCGGCCuuCCCGuCGuCCuCCGuCCCGCCGCCGCCGCCCACGACGACGGCGGCGCuCCCGCCGuuCCACGACuuCGCCuCCuCCAACCCGuuCGACGACGCGCCGCCCCCGuuCCuCGCGCCGCCGGGGCAGAAGCuCGGGuuCCuGGGGCCCCCCGGCGGCGCGuuCGGCGGCGGGAuGGGGuGGGACGACGACGACGAGAuCGAGCAGAGCGuGGACGCCuCGuCCAuGGGuGuCuCGGCCuCGCuGGAGAAuGCCGCGCCCGuGGCGGCCGGAGGAGGCGGCGGCGGuGGCGGCGGCGGuGGGAGGGGCAAGAAGAAGGGGAuGCCGGCCAAGAACCuCAuGGCGGAGCGGCGGCGCAGGAAGAAGCuCAACGACCGCCuCuACAuGCuGCGCuCCGuGGuGCCCAAGAuCAGCAAGAuGGACAGAGCuuCuAuCCuuGGuGAuGCAAuuGAGuACuuGAAGGAGCuCCuGCAGAGGAuuAACGAuCuuCACAAuGAACuuGAGuCuGCuCCuAGCuCuuCGCuuACuGGACCAuCAuCGGCuAGCuuCCACCCAuCAACACCCACAuuGCAGACGuuuCCuGGCCGCGuuAAGGAGGAACuuuGCCCAACCuCAuuuCCAAGCCCuAGuGGuCAACAAGCCACGGuCGAAGuuAGGAuGAGGGAAGGuCAuGCAGuCAAuAuCCACAuGuuCuGCGCACGCAGGCCCGGCAuuCuGAuGuCCACAuuGAGGGCuCuCGACAGCCuuGGCCuCGGGAuCGAGCAGGCGGuCAuCAGCuGuuuCAAuGGGuuuGCAAuGGAuGuuuuCCGCGCCGAGCAAuGCAGGGAuGGuCCuGGACuCGGGCCuGAAGAAAuuAAGACCGuGCuCCuGCACuCuGCuGGCCuuCAGAACGCAAuG
TFome clones do not contain stop codon at 3'end.
translation: MDEAEAAAAAAKMDELAGGGGGGGGDWSYLAADALAAASFTAFPFHHHHHHHHRDVLSASTPSSLLLNMDAATAAAMFDFQAAFPSSSVPPPPPTTTAALPPFHDFASSNPFDDAPPPFLAPPGQKLGFLGPPGGAFGGGMGWDDDDEIEQSVDASSMGVSASLENAAPVAAGGGGGGGGGGGRGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIEYLKELLQRINDLHNELESAPSSSLTGPSSASFHPSTPTLQTFPGRVKEELCPTSFPSPSGQQATVEVRMREGHAVNIHMFCARRPGILMSTLRALDSLGLGIEQAVISCFNGFAMDVFRAEQCRDGPGLGPEEIKTVLLHSAGLQNAM